烧伤小鼠早期应用帕瑞昔布钠对血清TNF-α的影响

Effects of early use of parecoxib sodium on serum TNF-αin mice with burn

目的探讨帕瑞昔布钠对烧伤小鼠血清中TNF-α水平的影响。方法雄性ICR小鼠200只随机均分为四组:IU组、TA组和TB组建立Ⅲ°烧伤模型后4h分别腹腔注射生理盐水、帕瑞昔布钠0.75mg/kg和帕瑞昔布钠7.5mg/kg;U组作为空白对照。帕瑞昔布钠首次给药后24h和48h重复给药。采用CBA液相芯片法检测给药后6h(T1)、12h(T2)、24h(T3)、48h(T4)和72h(T5)时的血清TNF-α浓度。结果与U组比较,IU组各时间点TNF-α均升高(P<0.05);TA组仅T1时TNF-α高于U组(P<0.05),而TB组T2-T5的TNF-α均低于U组(P<0.05)。与IU组比较,TA组T2-T5和TB组T1-T5的血清TNF-α均降低(P<0.05)。结论早期应用帕瑞昔布钠0.75mg/kg可适度降低烧伤小鼠血清TNF-α浓度,有利于抑制烧伤炎症的发展。而帕瑞昔布钠7.5mg/kg则过度降低了烧伤小鼠血清TNF-α浓度,不利于促炎因子与抑炎因子重归平衡。

Objective To observe the effects of early use of parecoxib sodium on serum tumor necrosis factor-α（TNF-α）in mice with burn.Methods Two hundreds ICR male mice were randomly divided into 4groups with 50 mice each.At 4hours after the burn models were established,the mice were intraperitoneally injected with normal saline（group IU）,parecoxib sodium 0.75 mg/kg（group TA）or parecoxib sodium 7.5mg/kg（group TB）,which was repeated at 24 and 48hours after the first injection.The mice without burn were taken as the controls（group U）.Serum levels of TNF-αwere detected by CBA liquid chip method at 6（T1）,12（T2）,24（T3）,48（T4）and 72hours（T5）after the first injection.Results Compared with group U,serum concentration of TNF-αin group IU at each time point was increased significantly（P〈0.05）.Serum level of TNF-αat T1 was higher in group TA than that in group U（P〈0.05）.Serum levels of TNF-αat T2-T5 were lower in group TB than those in group U（P〈0.05）.Serum levels of TNF-αat T2-T5 in group TA and at T1-T5 in group TB were lower than those in group IU（P〈0.05）.Conclusion The application of parecoxib sodium 0.75mg/kg in the early stage of burn can moderately decrease serum concentration of TNF-α,which is beneficial to inhibiting inflammation in mice with burn.But the use of parecoxib sodium7.5mg/kg may decrease serum concentration of TNF-αexcessively,which is not beneficial to keeping the rebalance of proinflammatory cytokines and anti-inflammatory cytokines.