水稻抗干尖线虫基因OC-XⅡ的克隆及表达研究

Cloning and Expression of the Gene OC-XII Conferring Resistance to Aphelenchoides besseyi in Rice

抗性验证试验表明籼稻大白谷抗水稻干尖线虫侵染。为进一步探究此抗性与病程相关蛋白——半胱氨酸蛋白酶抑制剂(Cystatin)的相关性,基于水稻基因组数据克隆籼稻大白谷Cystatin家族的OC-XII基因,分别进行OC-XII、JaponicaOC-XII和Indica-OC-XII蛋白质三维结构构建,并与水稻干尖线虫组织蛋白酶B(Cathepsin B)进行分子对接以验证OC-XII基因编码蛋白对Cathepsin B的抑制功能。通过荧光定量qPCR验证OC-XII基因在线虫侵染前后的表达特性,OC-XII蛋白可以与水稻干尖线虫Cathepsin B蛋白质特异结合,线虫侵染后OC-XII基因在浸染12h^2d期间表达量持续上调,侵染3d后下调。表明OC-XII基因在大白谷抗水稻干尖线虫侵染过程中发挥功能,具有对植物寄生线虫进行生物防治的潜力,有必要对其进行深入研究。

The previous resistance verification indicated that Dabaigu（Oryza sativa ssp.indica）resists the infection of Aphelenchoides bessey.To reveal the interaction between the resistance and the pathogenesis-related protein Cystatin,the gene OC-XII was cloned,the docking between OC-XII and A.besseyi Cathepsin B was done,and the expression of OC-XII was tested by quantitative real-time PCR.Results showed that OC-XII bound with Cathepsin B specifily.The expression level of OC-XII was upregulated from 12 hours after infection to 2days after infection,and downregulated 3days after infection.These results indicated that OC-XII was involved in the resistance to A.besseyi.The gene has the potential for biological control of plant-parasitic nematodes,and it is necessary to conduct in-depth research.