肌肽减轻H_2O_2诱导的N2a细胞损伤

Carnosine Reduces H_2O_2-induced cellular injury of N2a Cells

目的探究肌肽对H2O2诱导的N2a细胞损伤的保护作用及其机制。方法将N2a细胞分为对照组、损伤组(给予H2O2)和肌肽保护组(预先加入肌肽)。用相差显微镜观察了细胞形态,用免疫荧光染色和免疫印迹实验检测细胞AKT1和FAS-L的蛋白表达。结果肌肽显著增加了N2a细胞增殖能力(P<0.05);与对照组比较,H2O2损伤组细胞形态发生明显改变,细胞皱缩,胞膜变厚并出现发泡现象;而肌肽保护组细胞形态较损伤组明显改善;免疫荧光染色和免疫印迹实验显示,损伤组AKT1的表达显著低于对照组,FAS-L的表达显著高于对照组。而肌肽保护组AKT1的表达显著高于对照组,FAS-L的表达显著低于对照组(P<0.05)。结论肌肽能够减轻N2a细胞对H2O2诱导的细胞损伤。

Objective To investigate the protective effect of carnosine in oxidative stress induced by H2O2 by N2a cells. Methods The N2a cells were divided into three groups： control; H2O2group; carnosine group： carnosine was used in advance. Cell morphology was observed by phase-contrast microscope; immunohistochemistry and Western blot were applied to detect expression of AKT1 and FAS-L. Results Carnosine significantly promoted cell proliferation. Compared with control group,cell morphology changed significantly,cell shrinkage,membrane thickened,foaming phenomenon in H2O2 injury group; In the carnosine protection group,carnosine significantly inhibited the H2O2-induced cellular injury,cell morphology obviously better than H2O2 group. Compared with the control group,the expression of the AKT1 down-regulated and FAS-L up-regulated in H2O2 group,but in the carnosine protection group,the expression of the AKT1 was up-regulated and FAS-L down-regulated than H2O2group（ P〈0. 05）. Conclusions Carnosine obviously reduced H2O2-induced cellular injury of N2a cells.