巴西蕉诱导的香蕉枯萎病菌1号和4号小种致病相关基因表达分析

Pathogenicity-related Gene Expression Profile of Fusarium oxysporum f. sp. cubense Race 1 and Race 4 Induced by Musa AAA Cavendish Subgroup cv. Brazil

目前对于香蕉枯萎病菌的致病分子机制尚不十分清楚。为了阐明枯萎病菌的致病分子机制,从香蕉枯萎病菌1号(Foc1)和4号小种(Foc4)的比较蛋白质组学分析发现的表达量差异较大的蛋白中,选取了9个致病相关蛋白或潜在的致病基因,利用荧光定量PCR方法进行基因表达谱分析。结果显示:与对照相比,巴西蕉处理的Foc4中,上调表达的基因有:酰胺转移酶基因(amidotransferase)、线粒体过氧化物还原酶基因(mitochondrial peroxiredoxin)、几丁质酶基因(chitinnase 1)、羧肽酶基因(carboxypeptidase cpds),差异表达不明显的有腺苷激酶基因(adenosine kinase)、NADP-依赖型甘油脱氢酶基因(NADP-dependent glycerol dehydrogenase)、磷酸甘油酸激酶基因(phosphoglycerate kinase)、谷胱甘肽还原酶基因(glutathione reductase)、酰胺酶基因(amidase family protein)。Foc1中,与对照相比,上调表达的基因有:腺苷激酶基因、NADP-依赖型甘油脱氢酶基因,下调表达的基因有:酰胺转移酶基因、羧肽酶基因。综合分析显示,酰胺转移酶、过氧化物酶、几丁质酶、羧肽酶基因可能在Foc4的致病作用中起作用。

The understanding of Fusarium oxysporum f. sp. cubense molecular pathogenic mechanism is still not clear. Nine pathogenesis related protein genes or potential virulence genes were selected from lots of differences expression proteins discovered in comparative proteomic analysis of F. oxysporum f. sp. cubense race 1 and race 4and to analyze the gene expression profile by real time PCR. The results indicated that amidotransferase,mithchondrial peroxiredoxin, chitinnase 1, carboxypeptidase cpds were up-regulated compared with the controls in Foc4 group induced by Musa AAA Cavendish cv Brazil. Adenosine, NADP-dependent glycerol dehydrogenase,phosphoglycerate kinase, glutathione reductase and amidase family protein expressions were barely noticeable difference in two races. In Foc1, adenosine and NADP-dependent glycerol dehydrogenase were up-regulated in induced group compared with the control group. Amidotransferase and carboxypeptidase cpds were down-regulated.Those suggested that amidotransferase, mithchondrial peroxiredoxin, chitinnase 1 and carboxypeptidase cpds might play an important role in the pathogenesis of Foc4.