粘附分子CD62P和CD44在毛细支气管炎患儿外周血中的表达及意义

Expression and significance of adhesion molecules CD62P and CD44 in peripheral blood of infants with bronchiolitis

目的探讨黏附分子CD62P和CD44在毛细支气管炎(简称毛支炎)患儿外周血中的表达及意义。方法选取2014年11月至2015年5月住院治疗的毛支炎发病期患儿33例和恢复期患儿19例为研究对象,同期选取支气管肺炎患儿30例为支气管肺炎组,非感染患儿26例为对照组。采用流式细胞术检测各组患儿外周血CD62P的表达百分比,ELISA法测定血清CD44的水平。结果毛支炎发病期组CD62P和CD44水平显著高于毛支炎恢复期组、支气管肺炎组及对照组(P<0.05);毛支炎恢复期组CD62P和CD44的水平仍高于对照组(P<0.05)。毛支炎发病期患儿外周血中黏附分子CD62P百分比与血清CD44的水平呈正相关(r=0.91,P<0.05)。结论黏附分子CD62P、CD44参与了毛细支气管炎的发病过程,其水平高低可反映毛细支气管炎炎症反应的严重程度。

Objective To explore the expression and significance of the adhesion molecules CD62 P and CD44 in the peripheral blood of infants with bronchiolitis. Methods Thirty-three infants with bronchiolitis in the acute phase and 19 infants with bronchiolitis in the recovery phase, who were hospitalized between November 2014 and May 2015, were enrolled. Thirty infants with bronchopneumonia and 26 infants without infection were enrolled as the bronchopneumonia group and the control group, respectively. The CD62 P expression in the peripheral blood of each group was measured by flow cytometry, and the CD44 level in serum was determined using ELISA. Results The levels of the adhesion molecules CD62 P and CD44 in the bronchiolitis group in the acute phase were significantly higher than those in the bronchiolitis group in the recovery phase, the bronchopneumonia group, and the control group（P〈0.05）. The levels of the adhesion molecules CD62 P and CD44 in the bronchiolitis group in the recovery phase were also significantly higher than those in the control group（P〈0.05）. In the bronchiolitis group in the acute phase, there was a positive correlation between CD62 P expression and serum CD44 level（r=0.91; P〈0.05）. Conclusions The adhesion molecules CD62 P and CD44 play an important role in the pathogenesis of bronchiolitis, and their levels can reflect the severity of inflammatory response in infants with bronchiolitis.