摘要
转基因食品尤以植物源性转基因食品的安全性已成为公众关注的焦点。针对植物源性转基因食品甄别与安全性评估,已有多种检测手段得到广泛应用。PCR衍生技术因为具有高特异性、高敏感性等技术优势,为植物源性转基因食品的快速、准确检测提供了有效方法。PCR衍生技术包括多重PCR技术、实时荧光定量PCR技术、多重实时荧光定量PCR技术、多重巢式PCR技术以及多重PCR-DHPLC技术。本文就植物源性转基因食品检测的PCR衍生技术的研究进展作简要综述。
Safety of genetically modified food especially plant-derived genetically modified food are increasingly becoming attentive focus of the public. Many detection methods have been developed for the detection of discrimination and safe evaluation of plant-derived genetically modified food. PCR-derived technologies are sensitive and specific and so have provided rapid,accurate detection methods for plant-derived genetically modified food. PCR-derived technologies include multiplex PCR,real-time PCR,multiplex real-time PCR,multiplex nested PCR, multiple PCR- DHPLC. In this review, these PCR- derived methods should be summarized in brief.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第22期379-383,388,共6页
Science and Technology of Food Industry
基金
国家自然科学基金(31201901)
教育部留学回国人员科研启动基金(第46批
教外司留[2013]693号)
河南省教育厅科学技术重点研究项目(14A180025)
郑州市科技攻关项目(20130857)
郑州轻工业学院博士基金项目(2011BSJJ033)
郑州轻工业学院青年骨干教师资助计划项目(2013QNGG02)
郑州轻工业学院研究生创新基金(2014031)
关键词
植物源性
转基因食品
PCR
plant-derived
genetically modified food
PCR