樟树插穗对不同浓度牛血清白蛋白处理的反应

Response of Cinnamomum camphora Cuttings to Different Concentration of Bovine Serum Albumin Treatment

为探讨牛血清白蛋白(BSA)与植物扦插苗根系发育的关系,以园林植物樟树(Cinnamomum camphora)为试材,研究了不同浓度外源BSA对樟树扦插苗的生理和形态响应。结果表明:BSA浓度低于7.58μmol/L时,比对照提高了扦插苗的生根率、显著增加了侧根数和平均根长,使植物内源GA、ABA、ZR和IAA含量的变化向有利于根系形成和生长的方向变化,对扦插苗不定根形成有不同程度的促进作用。而高浓度的BSA(≥15.15μmol/L)比对照降低了扦插苗的生根率、显著减少侧根数和平均根长,使植物内源GA、ABA、ZR和IAA含量的变化向不有利于根系形成和生长的方向变化,对扦插苗不定根形成有不同程度的抑制作用。低浓度的BSA浸泡有利于插穗生根及根系的构建,而高浓度则相反。

In order to investigate the relationship between bovine serum albumin（BSA）and the development of plant root cuttings.The garden plant Cinnamomum camphora was used as experimental material,and the effect of different concentration of exogenous BSA on C.camphora cutting seedlings physiological and morphological response were studied.The results showed that the applying appropriate BSA（≤7.58μmol/L）was able to increase the cutting root rate and lateral root number and average length of root,enhance root activity and conducive the plant endogenous GA,ABA,ZR and IAA contents changed in the direction of promoting the growth of roots and adventitious root formation of cuttings.However,high concentration of BSA（≥15.15μmol/L）had different extent inhibition effect,and reduced the number of lateral roots,average root length and rooting rate than the control,and the plant endogenous GA,ABA,ZR and IAA contents changed in the direction of inhibiting adventitious root formation and the growth of root in different degrees.Low concentration of BSA soaking was conducive to rooting and root constructing,while high concentration on the contrary.