某食用白酒对大鼠血糖、胰岛素和胰高血糖素表达的影响

Effects of Liquor on Blood Glucose,Ins Expression and Glu Expression in Pancreatic Islet of Rats

目的:探讨某品牌食用白酒对大鼠空腹血糖、胰岛B细胞表达胰岛素(Ins)、A细胞表达胰高血糖素(Glu)的影响。方法:正常成年雄性SD大鼠40只,随机分为实验组(EG)和正常对照组(NCG);实验组30只又分为4周组(4 w)及12周组(12 w),每周组均分为低剂量组(L组)、中剂量组(M组)及高剂量组(H组),各实验组均有5只作正常对照;实验组大鼠予相应剂量食用白酒灌胃,每日11∶00 am及5∶00 pm各灌胃1次,正常对照组不予处理;分别于第4 w末及第12 w末测空腹血糖,采用免疫组化SABC法、图像分析及形态计量法计数各组大鼠胰岛Ins阳性细胞和Glu阳性细胞、平均光密度值及面数密度值。结果:各实验组大鼠空腹血糖水平与正常对照组比较,差异无统计学意义(P>0.05);各实验组Ins阳性细胞和Glu阳性细胞的分布、数量及免疫染色强度与正常对照组比较未见明显变化;与正常组比较,各实验组Ins阳性细胞、Glu阳性细胞平均光密度值差异无统计学意义(P>0.05),Ins阳性细胞、Glu阳性细胞的面数密度(NA)差异亦无统计学意义(P>0.05)。结论:某品牌食用白酒灌胃,在本实验设定的剂量和时程内,大鼠血糖未见明显异常,对胰岛B细胞表达Ins及NA、胰岛A细胞表达Glu和NA无明显影响。

Objective： To explore the effects of liquor（ 38% vol） on fasting blood glucose,Ins expression in pancreatic islet B cells and Glu expression in pancreatic islet A cells and to offer certain theoretical basis for studying healthy and scientific drinking. Methods： Forty normal male adult SD rats were randomly divided into normal control group and experimental group. Experiment group（ 30rats） were divided into 4 w group and 12 w group. The rats in different week group were further divided into low dose group（ L group）,middle dose group（ M group） and high dose group（ H group）.There were 5 normal control rats in 4 w group and 12 w group respectively. The rats in experiment group received intragastric administration of liquor twice a day,one at 11 ∶ 00 am and the other 5 ∶ 00 pm,while the normal control group remain no special treatment. At the end of 4 weeks and 12 weeks,fasting blood glucose was detected. Pituitary glands were excised at the end of 4 weeks,8 weeks and12 weeks. Immunohistochemical SABC,image analysis and morphometry methods were adopted to count Ins positive cells and Glu positive cells,mean optical density and NA. Result： There was no significant differences in fasting blood glucose level between experiment group and normal control group（ P〉0. 05）. Immunohistochemistry result showed that under the light microscope,the immunoreactive products of Glu positive cells and Ins positive cells were all brown particles in the cytoplasm. Morphological measurement results showed that there was no significant differences in the mean density and NA of Ins positive cells and Glu positive cells between experiment group and normal control group（ P〉0. 05）. Conclusion： The administration by gavage of this brand liquor（ 38% vol） with the dosage and the time period in this experiment has no significant effect on fasting blood glucose,Ins expression in pancreatic islet B cells and Glu expression in pancreatic islet A cells of rats.