FOC4拮抗放线菌DA07405发酵培养基的优化

Optimization of Fermentation Medium for Actinomycete DA07405 against FOC4

目的:提高放线菌DA07405产生抗古巴尖孢镰刀菌4号小种(FOC4)活性物质的能力。方法:通过单次单因子试验比较发酵培养基中几种常用碳氮源对DA07405发酵产生抗FOC4活性物质的影响,再采用正交试验获得DA07405发酵培养基中碳氮源的最佳配比组合;在最优碳氮源组合前提条件下,通过单因子实验获得DA07405发酵培养基中最适K2HPO4浓度。结果:获得最佳发酵培养基配方为:1.0%葡萄糖,0.5%蔗糖,0.5%玉米粉,0.75%大豆粉,0.05%K2HPO4;通过优化,DA07405发酵粗提物抑菌圈直径从15.0 mm提高到19.0 mm。结论:本研究结果为FOC4拮抗放线菌DA07405的工业化发酵生产提供了依据。

Objective： To improve the anti-FOC4 ability of DA07405 by producing secondary metabolite. Methods： Effect of several carbon and nitrogen sources on the anti-FOC4 activity of DA07405 were compared by using one-factor-at-a-time test,and then the optimum combination of carbon and nitrogen sources in DA07405 liquid fermentation medium was obtained by using orthogonal experiment.Under the optimum carbon and nitrogen sources,the optimum concentration of K2HPO4 was obtained using single factor experiment. Results： The results showed the optimum culture medium components contained 0. 05% K2HPO4,0. 5% corn powder,0. 5% sucrose,0. 75% soybean powder,1. 0% glucose; The antibacterial circle diameter of the crude extract of DA07405 increased from 15. 0 mm to19. 0 mm by optimized fermentation. Conclusions： This study provides a theoretical guidance for the industrial fermentation of actinomycete DA07405.