非酒精性脂肪肝大鼠肠道黏膜屏障功能的实验研究

Non-alcohol fatty liver disease reduces the intestinal barrier function of rats

目的肠道黏膜屏障功能紊乱与非酒精性脂肪肝(non-alcohol fatty liver disease,NAFLD)的发生关系密切,文中旨在通过动物模型的方法探讨NAFLD大鼠肠道黏膜屏障功能。方法 SD大鼠16只按随机数字表法分为正常组和NAFLD组,每组8只。正常组由普通饲料喂养,每100克普通饲料提供326 k Cal热量。NAFLD组大鼠由高脂饲料喂养,每100克高脂饲料提供453 k Cal热量建立NAFLD模型。采用HE染色检测肝病理,Elisa法和鲎试剂法分别检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1(Interleukin-1,IL-1)、内毒素,实时定量PCR法检测小肠闭锁小带蛋白ZO-1和连接蛋白Occluding的表达。结果 NAFLD组大鼠肝呈现典型的脂质沉积,血浆内毒素和血清TNF-α、IL-1较正常组显著增高,小肠ZO-1和Occluding表达较正常组显著降低(P<0.05)。结论 NAFLD大鼠存在肠道黏膜屏障功能下降。

Objective Disorder of intestinal barrier function is associated with the non-alcohol fatty liver disease（ NAFLD）.The present paper aimed to explore the intestinal barrier function in the rat model of NAFLD. Methods Sixteen SD rats were randomly divided into an NAFLD and a normal control group of equal number. The NAFLD models were constructed by high-fat feeding.HE staining was used for pathologic examination of the liver,the levels of TNF-α,IL-1,and endotoxin（ ET） were measured by ELISA and the limulus reagent method,and the expressions of intestinal ZO-1 and Occluding were determined by real time PCR. Results Compared with the normal controls,the NAFLD rats showed typical hepatic lipid deposition,with significantly increased levels of serum TNF-α and IL-1 and plasma ET and decreased expressions of intestinal ZO-1 and Occluding（ P 0. 05）. Conclusion Intestinal barrier function is decreased in NAFLD rats.