血清中期因子水平与糖尿病视网膜病变的相关性研究

Correlation between serum midkine level and diabetic retinopathy

目的探讨血清中期因子(Midkine)与DR的相关性。方法选取T2DM患者90例,分为T2DM不伴DR(NDR)组30例、T2DM伴非增殖期DR(BDR)组30例和T2DM伴增殖期DR(PDR)组30例。另选体检健康者30名作为健康对照(NGT)组,经75g OGTT排除糖尿病。检测各组FPG、HbA1c和血脂等指标,ELISA检测血清Midkine浓度。结果 T2DM各组血清Midkine水平均高于NGT组,Midkine水平由低到高依次为NGT<NDR<BDR<PDR组[(0.43±0.29)vs(1.73±0.25)vs(3.01±1.82)vs(4.82±3.62)pg/ml,P<0.05或P<0.01]。Pearson相关性分析结果显示,血清Midkine水平与DR病程、UAlb呈正相关(r=0.559、0.374,P<0.01)。多元逐步回归分析结果显示,DR病程为血清Midkine水平的独立影响因素(β=0.375,P<0.01)。结论血清Midkine水平与DR病程、DR严重程度密切相关,Midkine可能参与了DR发生发展,与DR关系密切。

Objective To investigate the correlation between serum midkine and diabetic retinopathy（DR）. Methods 90T2 DM were divided into four groups：T2DM patients without DR（NDR group,n=30）,T2 DM patients with background DR（BR group,n=30）,T2 DM patients with proliferative DR（PDR group,n=30）.Healthy subjects in routine examination were selected as normal control（NGT group,n=30）,confirmed by 75 g OGTT.FPG,HbA1 c and blood lipid were measured.Serum midkine level was determined by ELISA in each subject. Results Serum midkine level were higher in T2 DM groups than in NGT group.The order of serum midkine level from low to high were NGT group,NDR group,BDR group and PDR group[（0.43±0.29）vs（1.73±0.25）vs（3.01±1.82）vs（4.82±3.62）pg/ml,P〈0.05 or P〈0.01].Pearson correlation analysis showed that serum midkine level was positively correlated with duration of DR and UAlb level（r=0.559,0.374;P〈0.01）.Multiple stepwise regression analysis showed that duration of DR was an independent influencing factor for serum midkine level（β=0.375,P〈0.01）.Conclusion Serum midkine level is significantly associated with the duration and severity of DR.Midkine may be involved in the progression of DR.Thus there is a close relationship between midkine and DR.