脂联素及其受体2在肝细胞癌组织中的表达和临床意义

Expression of adiponectin and adiponectin receptor 2 in hepatocellular carcinoma tissue and its clinical significance

目的 探讨脂联素及其受体2在人肝细胞癌组织和癌旁组织中的表达及其与临床病理特征之间的关系。方法 选取武汉大学人民医院2012年7月~2014年7月手术切除的48例肝细胞癌患者和48例健康体检对照者作为研究对象,运用放射免疫法检测肝细胞癌患者和健康对照者的血清脂联素水平,采用免疫组织化学法检测脂联素及其受体2在人肝细胞癌组织和癌旁组织的表达,并统计分析肝癌组织中脂联素及其受体2的表达高低与临床病理特征之间的关系。结果 肝细胞癌患者的外周血清中脂联素水平为（12.25±5.23）μg/m L,低于健康对照者的（27.36±8.26）μg/m L,差异有高度统计学意义（P〈0.01）。脂联素在肝癌组织和癌旁组织中的阳性率分别为41.67%和81.25%,脂联素受体2在肝癌组织和癌旁组织中的阳性率分别为31.25%和77.08%,差异有高度统计学意义（P〈0.01）。进一步的分析发现,脂联素和脂联素受体2的阳性率与肝细胞癌患者的年龄、性别、HBs Ag、抗-HCVAb、是否合并有肝硬化、肿瘤大小、组织学分级和肿瘤分期均没有明显相关性,差异无统计学意义（P〉0.05）,但不同淋巴结转移情况和血管侵犯的肝癌组织中的脂联素和脂联素受体2的阳性率,差异有统计学意义（P〈0.05）。结论 脂联素及其受体2的表达下降可能与人肝细胞癌的发生发展密切相关。

Objective To investigate the expression of adiponectin and adiponectin receptor 2 （AdipoR2） in hepatocellular carcinoma （HCC） tissue and para-carcinoma tissue, and their correlation to clinicopathological features. Methods Forty-eight HCC patients undergoing partial hepatectomy and forty-eight healthy people in Renmin Hospital of Wuhan University from July 2012 to July 2014 were selected as research objects. Serum adiponectin level of HCC patients and normal controls was determined using RIA kit. The expression of adiponectin and AdipoR2 in cancerous and para- cancerous tissues were detected by immunohistochemical method. The correlations of clinical pathologic parameters with the adiponectin and AdipoR2 expression in cancerous tissue were analyzed respectively. Results HCC patients had a significantly lower concentration of serum adiponectin than nomal controls [（12.25±5.23） vs （27.36±8.26） μg/mL, P 〈 0.01]. The expression rate of adiponectin and AdipoR2 in cancerous tissues were lower than those in the para-cancerous tissues obviously （41.67% vs 81.25%, 31.25% vs 77.08%, P 〈 0.01）. There were no statistical differences of the expression of adiponectin and AdipoR2 in age, sexuality, HBsAg, anti-HCV Ab, liver cirrhosis, tumor size, histological differentiation degrees and stages of TNM, but there were statistical difference of the expression of adiponectin and AdipoR2 with vascular invasion and lymphatic metastasis （P 〈 0.05）. Conclusion The decrease of adiponectin and AdipoR2 expression may be closely relevant to the HCC progression.