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大鼠发育过程中外毛细胞对GABA的反应

The response of GABA eliciting the rats outer hair cells during development
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摘要 目的:用电生理方法验证GABA能诱使SD大鼠发育中外毛细胞产生反应,并为外毛细胞上有GABA受体提供证据及研究GABA作用与发育期外毛细胞之间的关系。方法:全细胞记录顶部外毛细胞在GABA作用下膜电位及电流的变化。然后电生理数据进行统计,电生理数据中GABA能引起细胞发生膜电位变化或电流变化占所记录细胞的比例,并以此来分析其与发育之间的关系。结果:电生理数据显示GABA能使细胞发生超极化或产生内向电流,而且细胞反映百分数也是随发育逐渐减少的。结论:GABA引起外毛细胞的作用随发育逐渐减少,这意味着该递质在外毛细胞发育过程中可能有牵引传出纤维到达外毛细胞区域或促进MOC-OHC突触形成的作用。 Objective：We used electrophysiological methods to study that whether GABA could elicit OHCs outward currents provide evidence for exsitence of GABA-A receptor and investige the relationship between the effect of GABA and the development of OHCs.Method：We used whole-cell recording OHCs at current-clamp or voltage-clamp to verify the function of GABA receptor on OHCs.Then we counteds the responsive cells vs.total number cells,and according to results to study the relationships between the GABA receptor and development of OHCs.Result：OHC was elicited outward current or hyperpolarized by GABA and the responsive cells were decreased with development.Conclusion：The result of GABA receptor decreasing with development suggested that the receptor may draw efferents to OHCs or facilitate the MOC-OHC synapse formation.
作者 冯伟伟 唐杰
机构地区 南方医科大学基础医学院生理学教研室
出处 《临床耳鼻咽喉头颈外科杂志》 CAS 北大核心 2015年第22期1999-2002,共4页 Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金 国家自然科学基金(No:31271179) "973"项目(No:2014CB943002)
关键词 GABA 外毛细胞 发育 电生理 GABA outer hair cell development electrophysiology
分类号 R764 [医药卫生—耳鼻咽喉科]
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参考文献15

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二级参考文献10

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临床耳鼻咽喉头颈外科杂志
2015年 第22期

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