硫酸软骨素对LLO诱导大鼠肠黏膜微血管内皮细胞分泌NO、ET-1的影响

Effects of Chondroitin Sulfate on the Secretion of NO and ET-1 in Rat Intestinal Mucosa Microvascular Endothelial Cells Induced by LLO

为研究李斯特菌溶血素(LLO)对大鼠肠黏膜微血管内皮细胞(RIMVECs)分泌NO、内皮素(ET-1)的影响,以及硫酸软骨素(CS)通过调节细胞微环境保护RIMVECs的作用,将体外培养的RIMVECs分为LLO+CS组、LLO组、CS组、空白组,经过MTT比色法测定细胞生长情况,硝酸还原酶法测定细胞上清液细胞因子NO含量以及酶联免疫法检测ET-1含量,并用原位杂交方法对结果进行验证。结果表明:LLO可抑制RIMVECs增殖活性,提高NO、ET-1分泌量且高于正常水平,并导致NO/ET-1比值失衡(下降);而CS可提高RIMVECs细胞的增殖活性,并显著上调NO/ET-1比值。由此可知,CS可通过改善LLO引起细胞因子NO和ET-1失衡而起到保护微血管内皮细胞的作用。

The purpose of this study was to investigate the treatment mechanism of the chondroitin sulfate（CS） on the listeriosis and the secretion of NO and ET- 1 in rat intestinal mucosa microvascular endothelial cells（RIMVECs） induced by listeriolysin O（LLO）.RIMVECs in culture were divided into LLO ＋ CS,LLO,CS and blank control groups.Cells were assessed proliferation by MTT assay.The Changes of NO and ET- 1 level were measured by the method of nitratase,ELISA and hybridization in situ.Compared with other groups,proliferation of LLO group was very significantly decreased.The production of NO and ET- 1 were enhanced for 12 h,and NO/ET- 1 ratio was lower.CS could regulate imbalance of NO/ET- 1 induced by LLO and improve the local microcirculation of intestine.