青蒿素联合硫酸亚铁对小鼠移植乳腺癌MT40作用

The Effects of Artemisinin Combined with Ferrous Sulfate on Transplanted MT40 Cells of Mice

目的探讨青蒿素联合硫酸亚铁对小鼠移植乳腺癌MT40作用。方法建立小鼠移植乳腺癌MT40动物模型，将造型成功30只小鼠随机分成5组，每组6只，即青蒿素实验组、硫酸亚铁对照组、青蒿素联合硫酸亚铁组、阳性对照组（丝裂毒素组）、阴性对照组。制作青蒿素联合硫酸亚铁、硫酸亚铁、青蒿素、阴性对照、阳性对照组小鼠移植乳腺癌MT40荷瘤鼠肿瘤生长曲线，计算各组肿瘤体积倍增时间；计算各组抑瘤率；TUNEL检测各组肿瘤组织细胞凋亡率。结果与阴性对照组相比，各组小鼠移植乳腺癌MT40生长曲线受到抑制。与阴性对照组比较，青蒿素实验组抑制肿瘤的生长，抑瘤率42．2％（P〈0．05），阳性对照组也能抑制肿瘤生长，抑瘤率为44．6％（P〈0．01），青蒿素联合硫酸亚铁实验组抑制肿瘤生长作用更加显著，抑瘤率为55．1％（P〈0．05）。肿瘤体积倍增时间延长，各组瘤体倍增时间为：阴性对照组2．77d，硫酸亚铁对照组2．71d，青蒿素实验组3．45d，青蒿素＋硫酸亚铁组3．86d，阳性对照组3．44d。TUNEL检测各实验组肿瘤组织细胞凋亡，阴性对照组及硫酸亚铁对照组可见零星细胞凋亡，青蒿素实验组、青蒿素联合硫酸亚铁实验组及阳性对照组见较多细胞凋亡，青蒿素实验组肿瘤细胞凋亡率21．23％，青蒿素联合硫酸亚铁实验组肿瘤细胞凋亡率34．25％，阳性对照组肿瘤细胞凋亡率为17．42％。结论青蒿素联合硫酸亚铁对小鼠移植MT40具有明显抑制、诱导凋亡作用。

Objective To explore the effects of artemisinin combined with ferrous sulfate on transplanted MT40 cells. Methods Transplanted MT40 mice model was established. 30 modeling mice were divided into 5 groups ： arte- misinin group, ferrous sulfate group, artemisinin combined with ferrous sulfate group, negative control group, positive control group. The growth curves of transplanted MT40 were manufactured for every group. Tumor doubling time and tumor inhibition rate were calculated. Cell apoptosis rates were detected by TUNEL. Results Compared with negative control group, growth curves were suppressed in the other groups. Tumor inhibition rate in artemisinin group was 42.2% （ P 〈 0.05 ）. Tumor inhibition rate of mitomycin was 44.6% （ P 〈 0.01 ）. Tumor inhibition rate in artemisinin combined with ferrous sulfate group was 55.1% （P 〈 0.05 ）. Tumor doubling times were prolonged in negative control group（2.77 d）, ferrous sulfate group（2.71 d）, artemisinin group（3.45 d）, artemisinin combined with ferrous sul- fate group（3.86 d）, positive control group（3.44 d）. Cell apoptosis in negative control group and ferrous sulfate group was scattered. Cell apoptosis rates in artemisinin group, artemisinin combined with ferrous sulfate group and pos- itive control group were 21.23%, 34.25% and 17.42% respectively. Conclusion Artemisinin combined with fer- rous sulfate can inhibit growth and induce apoptosis of transplanted MT40 cells.