拟松材线虫组织蛋白酶基因Bmcath1的序列分析与转录水平检测

Bioinformatic and expression analysis of a cathepsin gene Bmcath1 in Bursaphelechus mucronatus

拟松材线虫组织蛋白酶基因Bmcath1是一个在松材线虫与拟松材线虫中差异表达的基因,为了解该基因的生物学功能,对Bmcath1基因进行了生物信息学分析。笔者使用BioEdit、SignalP 4.1、BLASTP、MEGA 4.0等软件对Bmcath1基因进行了生物信息学分析,发现Bmcath1 cDNA序列全长1 294 bp,含有一个长度为1 158 bp的开放阅读框。由Bmcath1基因编码的蛋白质CATH1含有386个氨基酸,分子质量为4238 ku,等电点为8.52。蛋白质CATH1存在一个Peptidase_C1A subfamily功能域结构,在不同物种上的氨基酸序列多样性丰富。采用Real-Time PCR方法对不同培养方式获得的拟松材线虫中Bmcath1基因的表达量进行检测,发现该基因在分离自黑松的拟松材线虫中高量表达,据此推测拟松材线虫Bmcath1基因可能在线虫处于松树体内生长、繁殖时发挥更为重要的作用。

Cathepsin geneBmcath1 ofBursaphelenchus mucronatusis differentially expressed betweenB. xylophilusandB. mucronatus. In order to understand its biological function, bioinformational analysis was conducted using software Bi-oEdit, SignalP 4.1, BLASTP and MEGA 4. 0. The result showed that the full-length cDNA ofBmcath1 was 1 294 bplong, which included an ORF of 1 158 bp. The deduced amino sequence of CATH1 contained 386 amino acids with mo-lecular mass of 42.38 ku and pI of 8.52. Protein CATH1 contained a Peptidase-C1 A conserved domain. The amino se-quences of cathepsins varied between different species. With the method of real-time PCR, gene expression profilingshowed thatBmcath1 expressed much more inB. mucronatusseparated fromPinus thunbergiiseedlings comparing withexpression level ofBmcath1 in monoxenic culture nematodes. The result suggested that function ofBmcath1 may be relat-ed to infection process ofB. mucronatus.