超弱发光技术评价脉冲电场对玉米萌发期抗旱性的影响

Using Ultraweak Luminescence Techniques to Evaluate the Effect of Pulsed Electric Field on Drought Resistance of Corn during Germination

为了从活体细胞角度阐述脉冲电场对作物萌发期抗旱性的影响及其机理,采用渗透势为-0.1 MPa的PEG-6000溶液形成干旱胁迫,研究了场强为100kV/m、脉冲宽度为80ms、脉冲频率为1Hz的脉冲电场处理对干旱胁迫下萌发的玉米种子自发发光和延迟发光的影响.结果表明,在玉米萌发过程中,自发发光和延迟发光积分强度逐渐增长,而在干旱胁迫下降低,脉冲电场处理使干旱胁迫下萌发玉米种子的自发发光强度和延迟发光积分强度有所回升.自发发光强度和延迟发光积分强度的变化与玉米萌发抗旱指数和贮藏物质转运率的变化一致,表明脉冲电场处理提高了玉米萌发期的抗旱性,萌发玉米自发发光和延迟发光积分强度的变化分别从细胞呼吸代谢和细胞伤害的角度反映了脉冲电场对萌发玉米抗旱性的影响.研究结果揭示了脉冲电场对作物萌发期抗旱性的影响机理,可对开发基于脉冲电场的农业物理新技术提供参考.

In order to clarify and evaluate the effect of pulsed electric field on drought resistance of maize during germination and its mechanism based on living cells,the-0.1 MPa osmotic potential of PEG-6000 solution was used to form drought stress,the effect of pulsed electric field treatment with field strength of 100kV/m,width of 80 ms,pulse frequency of 1Hz on spontaneous luminescence and delayed luminescence from germinating corn seeds under drought stress were studied.The results show that the spontaneous luminescence and delayed luminescence integrated intensity gradually increase during the germination process of corn and decrease cased by the spontaneous luminescence,pulse electric treatment makes delayed luminescence integrated intensity of germinating corn under drought stress increase,the changes of the spontaneous luminescence and delayed luminescence integrated intensity are same as the changes of the germination drought index and the storage material transport rate of germinating corn.These results indicated that the treatment of pulsed electric fields improves drought tolerant of germination corn.The changes of the spontaneous luminescence and delayed luminescence integrated intensity of germinating corn reflecte the effect of pulsed electric field treatment on the drought resistance of germinating corn from cell metabolism and cell damage respectively.The research results reveal themechanism of pulsed electric field on the drought resistance of germinating crop seeds,and provide a reference for development of agricultural technology based on pulsed electric field.