芍药苷预处理激活PPARα对在体大鼠心肌缺血再灌注损伤保护的作用研究

Effects of Paeoniflorin Pretreatment by Activating PPARα on the Rats with Myocardial Ischemia Reperfusion Injury

目的探究芍药苷预处理通过激活过氧化物酶体增殖物激活受体(PPARα)信号通路对大鼠在体心肌缺血再灌注损伤发挥的干预作用。方法采用结扎SD大鼠心脏LDA的方法建立在体大鼠心肌缺血再灌注损伤模型,利用0.5%Evan’s蓝灌注心肌及1%TTC磷酸缓冲液孵育心肌来观察心肌坏死利用HE染色观察心肌损伤程度,利用酶联免疫吸附法观察大鼠血清中肿瘤坏死因子(TNF-α)、细胞黏附因子(ICAM-1)的含量,蛋白印迹(Western blot)技术检测芍药苷预处理对PPARα的影响。结果芍药苷药物预处理组与缺血再灌注组比较,心肌梗死面积明显减小(P<0.01);芍药苷预处理能促进PPARα蛋白的表达,减少血清中TNF-α、ICAM-1的含量。结论芍药苷预处理能减轻大鼠在体心肌缺血再灌注损伤,可能通过增加PPARα蛋白的表达,抑制TNF-α、ICAM-1分泌,减少炎症反应,进而发挥保护作用。

Objective： To explore the effects of paeoniflorin preconditioning by activating PPARα on the rats with myocardial ischemia reperfusion injury. Methods： Myocardial ischemia reperfusion injury model was estab lished by cardiac LDA ligation in SD rats. The degree of myocardial injury was observed by perfusion of 0.5%Evan′s blue and 1% TTC. HE staining was used to observe the degree of myocardial injury. The content of TNF-α and ICAM-1 in serum were detected with ELISA（Enzyme-Linked Immunosorbent）.Western blot technology was used to explore the effects of paeoniflorin pretreatment on PPARα. Results： In comparison with ischemic reperfusion group,paeoniflorin pretreatment could decrease infarction area significantly（P〈0.01）,reduce the contents of TNF-α and ICAM-1 significantly（P〈0.05） and increase the expression of PPARα. Conclusion： The effect of paeoniflorin preconditioning on rats with myocardial ischemia reperfusion injury may be probably related to the increase of the expression of PPARα and the decrease tof he contents of TNF-α and ICAM-1 in serum