刮痧联合放血对热射病大鼠下丘脑HSP-70mRNA及蛋白的影响

Effect of Combination Treatment with Guasha and Blood-letting on the Expression of HSP-70 in the Hypothalamus of Rats during Heat Stroke

目的观察背部刮痧结合放血对热射病模型大鼠的影响,检测下丘脑热休克蛋白-70(HSP-70)mRNA及蛋白表达的变化。方法雄性SD大鼠80只,随机分为常温对照组,热应激组,刮痧组,放血组和刮痧结合放血组,每组16只。监测治疗前后动物心率、直肠温度、平均动脉压的动态变化及生存时间。致热休克后20 min后蛋白印迹法和实时荧光定量PCR法观察大鼠下丘脑HSP-70 mRNA及蛋白的表达变化。结果热应激组大鼠存活时间为(23±2)min,刮痧组、放血组及刮痧结合放血组均延长了热应激大鼠的存活时间(P<0.01),以刮痧结合放血组更显著(P<0.01)。与常温对照组比较,热应激后下丘脑HSP-70 mRNA及蛋白表达升高(P<0.01);刮痧组、放血组、刮痧结合放血组后HSP-70 mRNA及蛋白表达均上调,与热应激组比较差异有统计学意义(P<0.01);以刮痧结合放血组HSP-70 mRNA及蛋白的表达上调更显著(P<0.01)。结论对热射病模型进行背区刮痧结合放血治疗可延长生存时间,可能通过上调HSP-70水平,减轻病理损伤而起到治疗作用。

Objective： To assess the therapeutic effect of Guasha on the back combined with blood-letting at EX-UE11（shixuanon） on heat stroke. Methods： 80 male SD rats,immediately after the onset of heatstroke,were divided into 5 groups： the control group,the heat stress group,the Guasha group,the blood letting group and the combination group of Guasha and blood-letting,with 16 rats in each. The dynamic changes of heart rate,rectal temperature,mean arterial pressure and survival time were detected before and after treatment. 20 minutes after heat stoke,the expressed variation of HSP-70 protein were detected by Western blotting and measured by RT-q PCR. Results： In the heat stress group,their survival time was found to be 21 to 25 minutes. The survive time was also extended in the the Guasha group,the blood letting group and the combination group of Guasha and blood-letting,especially in the combination group of Guasha and blood-letting. Compared with the control group,there were increased expressions of HSP-70 gene and protein in the other 4 groups（P〈0.01）. There was significant difference among the other 4 groups in the expressions of HSP-70 gene and protein（P〈0.01）. Conclusion：The results indicate that the resuscitation of Guasha combined with blood-letting at the time point of onset of heat stroke may improve survival. The expressions of HSP-70 gene and protein in the hypothalamus of rats are closely related to the pathologic development of heat stroke.