穴位注射骨瓜提取物注射液联合电针夹脊穴对兔骨质疏松性椎体压缩骨折二聚糖的影响

Effect of Acupoint Injection of Bone Extract Combined with Electro Acupuncture on the Biglycan of the Osteoporotic Vertebral Compression Fractures

目的观察穴位注射骨瓜提取物注射液联合电针夹脊穴对骨质疏松性椎体压缩性骨折动物模型二聚糖及血清基质金属蛋白酶(MMPs)的影响。方法雌性新西兰兔40只,随机分为模型组、穴位注射组、电针组、联合治疗组,采用去势法手术切除新西兰兔卵巢,用混有糖皮质激素的饲料饲养4个月后造成动物骨质疏松,再采用手术方法造成骨质疏松性椎体压缩骨折动物模型,造模成功后电针组电针夹脊穴30 min;穴位注射组选夹脊穴多点穴位注射骨瓜提取物注射液;联合治疗组电针夹脊穴30 min后穴位注射骨瓜提取物注射液。采用酶免疫吸附试验测定血清基质金属蛋白酶(MMPs:MMP-1、MMP-2、MMP-3、MMP-9、MMP-10)阳性表达率,采用免疫荧光法测定腰椎间盘组织二聚糖中的表达。结果经21 d治疗,联合治疗组腰椎间盘组织二聚糖表达明显增高,血清MMP-1、MMP-3、MMP-9明显降低,与穴位注射组及电针组比较,差异均有统计学意义(P<0.05)。结论穴位注射骨瓜提取物注射液联合电针夹脊穴治疗兔骨质疏松性椎体压缩性骨折的作用机制可能为电针夹脊穴可激发经气、活经通络、提高二聚糖黏附椎间盘细胞数量、调节椎间盘成骨细胞及破骨细胞的动态平衡,骨瓜提取物注射液穴位注射吸收后降低MMP-1、MMP-3、MMP-9对腰椎间盘基质的降解,抑制椎间盘组织退行性变,调节退变椎间盘细胞的代谢平衡,促进骨折愈合的作用。

Objective： To establish an animal model of osteoporotic vertebral compression fractures in New Zealand rabbits,and observe the effect of acupoint injection combined with electro acupuncture and electro acupuncture on the treatment of these diseases,and provide a theoretical basis for the clinical treatment of this disease. Methods： 40 female New Zealand rabbits were randomly divided into the model group,the acupoint injection group,the electroacupuncture group,the combined treatment group,with castration method to remove the ovary of New Zealand rabbit,fed with mixed sugar cortical hormone feed for 4 months,causing osteoporosis. After making a successful model,the EA group received EA clip ridge point for 20 minutes;the acupoint injection group selected Jiaji point acupoint injection of bone melon extract injection;the combined treatment of electroacupuncture group received Jiaji acupoint injection and 30 minutes later the bone melon extract injection. The positive expression rate of serum matrix metalloproteinases（MMPs metallopro-teinases,MMPs：MMPs MMP-1,MMP-2,MMP-3,MMP-9,and MMP-10） were detected with Enzyme immunoassay method,and the expression of biglycan in lumbar intervertebral disc tissue was tested with immune fluorescence method. Results： After 21days′ treatment,compared with the acupoint injection group and the electroacupuncture group,the expression of biglycan in lumbar intervertebral disc tissue of the combined treatment group increased significantly with statistical significance（P〈0.05）. Conclusion： The mechanism of the treatment may be that EA Jiaji points can arouse the Qi,Tongluo,increase the number of biglycan adhesion of intervertebral disc cells,regulate the dynamic balance of the intervertebral disc cells and osteoclasts and that bone melon extract injection in acupoint injection of absorption decreases MMP-1,MMP-3,MMP-9 on lumbar intervertebral disc matrix degradation,inhibit intervertebral disc degenerative changes and regulate metabolic balance of degenerated intervertebral disc cells and promote fracture healing.