摘要
目的探讨中成药血塞通(Xuesaitong,XST)对全脑缺血/再灌注后大鼠海马回中凋亡基因Bcl-2及Bax的表达影响,以及可能对全脑缺血/再灌注损伤的保护作用。方法 1采用Pulsinelli等的方法建立大鼠急性全脑缺血/再灌注型;2分别于再灌注后3、6、12、24和48 h 5个时间点处死大鼠取出脑组织,固定切片,采用免疫组化法检测海马CA1区Bcl-2和Bax的表达,TUNEL染色检测凋亡细胞;3对90只健康实验大鼠分为三组即假手术(SO)组;脑缺血/再灌注(I/R)组;血塞通(XST)组,对三组进行比较分析,研究血塞通对Bcl-2和Bax表达的影响。结果脑缺血/再灌注期间,I/R组锥体细胞Bcl-2和Bax均比SO组的表达平均灰度值低。各时间点平均灰度值比较,差异有显著性(P<0.01),体内锥体细胞凋亡率增高(P<0.01);XST组与I/R组相比较,Bcl-2表达平均灰度降低,Bax表达平均灰度增高,各时间点表达平均灰度值比较均有统计学意义(其中第3 h时间点P<0.05,余各时间点P<0.01),体内锥体细胞凋亡率降低(P<0.01)。结论大鼠全脑缺血/再灌注损伤后,血塞通通过上调Bcl-2蛋白的表达,下调Bax蛋白的表达,抑制细胞凋亡发挥神经保护作用。
Objective To investigate the expression of Bcl-2/Bax and apoptosis on global cerebral ischemic-reperfusion injury in rats after xuesaitong （XST） intervention therapy. Methods The cerebral ischemia reperfusion model was established by the method of Pulsinelli. A total of 90 healthy SD rats were randomly divided into 3 groups, including sham operation （SO） group （n=30）, ischemic reperfusion （I/R） group （n=30） and XST group （n=30）. 3, 6, 12, 24, 48 h after cerebral ischemia-reperfusion, rats in xuesaitong group received the XST intervention therapy （50 mg·kg-1·6h-1）;In the meantime, rats in SO group and I/R group received the saline intervention therapy. Bcl-2 and Bax expression in rat hippocampal CA1 pyramidal cells were assayed by immunohistochemical staining. TUNEL staining was performed to detect the number of surviving neurons. Results In I/R group, the average gray value of Bcl-2 and Bax decreased （P〈0.01）, and the number of surviving neurons reduced （P 〈0.01） compared with SO group;In XST group, the average gray value of Bcl-2 decreased （P〈0.01） and Bax increased （P〈0.05）, and the number of surviving neurons increased （P〈0.01） compared with SO group. Conclusions Xuesaitong has protective function after cerebral ischemia-reperfusion by regulating the expression of Bcl-2 protein, downregulating the expression of Bax, and inhibiting the apoptosis.
出处
《临床医学工程》
2015年第11期1426-1428,共3页
Clinical Medicine & Engineering
基金
深圳市宝安区科技局科研立项(项目编号:2013131)
