MiR-36f对猪蛔虫幼虫感染力和发育的影响（英文）

Effects of miR-36f on larval infection and development of Ascaris suum

目的探讨猪蛔虫虫卵和幼虫期特异表达的miR-36f的是否与猪蛔虫幼虫的感染性和发育有关。方法将合成的miR-36f的模拟物,抑制剂以及模拟物对照和抑制剂对照通过浸泡的方法被猪蛔虫三期幼虫吸收后,分别将这四组幼虫通过灌胃的方式感染小鼠,以不作任何处理的幼虫感染小鼠作为空白对照组,四天后采用贝尔曼法收集各组小鼠肺和肝的幼虫。对各组回收幼虫的数量以及幼虫的长度和宽度进行测量,并采用qRT-PCR方法对miR-36f的两个靶基因OST48和cytochrome b进行定量分析。结果抑制miR-36f以后,miR-36f的两个靶基因OST48和cytochrome b均被下调,幼虫发育和感染力受到抑制。结论提示猪蛔虫的miR-36f与幼虫个体发育和感染力有关。

MicroRNAs （miRNAs） are key regulators of gene expression. The miR-36 family has only been authentically found in helminth including Ascaris spp. , Caenorhabditis elegans, Brugia rnalayi, and Schistosoma mansoni. And the family member named miR-36f was identified to exist only in the egg and larval stage of Ascaris suum （A. suurn） in our previous stud- y. We herein preliminarily evaluated the function of miR-36f via its mimics and inhibitors by using A. suum as model organism. The miR-36f mimics, inhibitors, mimics negative controls and inhibitor negative controls were absorbed by the different groups larvae via soaking method. Three days later, the mice were infected with the larvae above. And the larvae were recovered from the liver and lung of the mice respectively. The larvae of each group were counted and their length and width were measured, and the two target genes of miRNA were analyzed by qRT-PCR. The results showed that development and infective numbers of the A. suum infective larvae were significantly influenced by miR-36f inhibitors （P^0.05）, when compared with the mimics （P^0.05）. The expression of the two key target genes of miR-36f named OST48 and cytochrome b were similar to that of in- hibitor and mimics. When them iR-36f was influenced by its inhibitor, the development and infective ability of A. suurn larvae were reduced, which indicated that the miR-36f might be associated with development and infectivity of the A. suurn larvae. Furthermore, miR-36f inhibitors seemed more effective than its mimics. The results would be helpful for better understanding and novel control strategy for parasitic helminths.