环介导等温扩增技术及其在病原生物检测中的应用

Loop mediated isothermal amplification and its application in pathogenic organisms detection

环介导等温扩增(LAMP)技术是近年来发展起来的一种新型核酸扩增技术,具有操作简便、快速、特异性高、灵敏性高等特点,自2000年开发以来,在短短的十几年内被广泛应用于细菌、病毒和寄生虫等病原生物的检测,在即时检验和基层实验室推广中有重要意义。

Loop mediated isothermal amplification （LAMP） technology is a novel nucleic acid amplification technology de- veloped in recent years. Because of its simplicity, rapidity, high specificity and high sensitivity, it has been widely used in the detection of pathogenic organisms such as bacteria, viruses and parasites in the past dozen years since 2000, it has important significance of point-of-care test and local laboratory promotion.