摘要
目的对丽江市古城区的一起鼠间鼠疫进行判定。方法对1份干燥自毙大绒鼠标本进行鼠疫反相间接血凝抑制试验(RIHA)、聚合酶链式反应(PCR)及实时荧光定量PCR(RT-PCR)检测。结果该标本经免疫学检测、PCR及RT-PCR检测均为阳性。结论确认丽江市古城区鼠间鼠疫疫情。
To determine a rat plague epidemic in ancient city administrative region of Liiiang, China, one of drying self-dead Eothenomys miletus were detected with methods of reverse indirect hemoagglutination inhibition test (RIHA), Polymerase Chain Reaction (PCR) and real-time PCR (RT-PCR). Results showed that the immunological detection, PCR and RT-PCR de- tection to this sample were positive. It was confirmed that a rat plague epidemic occurred in ancient city administrative region of Lijiang.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2015年第11期1086-1088,共3页
Chinese Journal of Zoonoses
关键词
鼠疫
实时荧光定量PCR
自毙鼠
丽江市古城区
plague
real time fluorescence quantitative PCR (RT PCR)
self-dead rodent
ancient city administrative region of Lijiang