紫外线光照射促进微弧氧化纯钛诱导磷灰石形成及细胞矿化

In vitro study on the effect of ultraviolet irradiation on induction apatite formation and cell mineralization of micro-arc oxidized titanium surface

目的评估紫外线照射效应对微弧氧化纯钛诱导磷灰石形成及细胞矿化的影响。方法Ⅱ级商用纯钛片进行微弧氧化(MAO)处理,作为空白对照组,记为MAO;微弧氧化结合紫外线(UV)照射处理2 h的钛片作为实验组,记为MAO+UV 2 h。采用扫描电镜(SEM)观察钛片浸泡在DMEM/F12培养液中材料表面磷灰石形成情况;将钛片与大鼠脂肪间质干细胞矿化诱导条件下共培养,观察细胞矿化情况。结果浸泡在培养液中21 d后,MAO组仅在SEM高倍镜下见少量散在微小磷灰石结晶颗粒;MAO+UV 2 h组颗粒明显增大增多且叠层沉积,除微弧氧化形成的孔径外周高点外,氧化膜层几乎全部被磷灰石晶体层覆盖,且孔径内沉积大量晶体使孔径明显缩小。SEM观察细胞矿化物的形成情况,发现MAO组未见明显矿化物形成,但MAO+UV 2 h组细胞材料表面可见较多针状结晶,能谱分析(EDS)提示为磷灰石结晶。结论紫外线照射可增强微弧氧化纯钛表面在体外诱导磷灰石沉积的能力且促进脂肪间质干细胞的体外矿化。

Objective To evaluate the effect of ultraviolet irradiation on induction apatite forma？tion and cell mineralization of micro？arc oxidized titanium surfaces,using cell culture medium immersion tests and interactions between adipose derived mesenchymal stem cells and material surfaces. Methods Pure titanium disks were treated by micro？arc oxidation（MAO）,as blank control group,marked as MAO. MAO combined with ultraviolet irradiation treatment for 2 hours,as treatment group,marked as MAO＋UV 2 h. Apatite formation on the surface of titanium disks and the cell mineralization were observed by a scanning electron microscopy（SEM）after immersed in DMEM/F12 medium and cultured with rat adipose derived mesenchymal stem cells（AMSCs）. Results Immersed in medium for 21 days,few scattered tiny apatite crystal particles were found in MAO group;while in MAO＋UV 2 h group,increasing size and lami？nated deposition of apatite crystal particles could be observed. Almost the whole oxide layer was covered by them,and some deposited in the aperture of the titanium surface. After AMSCs cultured,no mineral com？pound formation was found in MAO group;however,in MAO＋UV 2 h group,lots of needle crystals formed and EDS result suggested them as apatite crystals. Conclusion In vitro ultraviolet irradiation can promote the abilities of induction apatite formation and cell mineralization of micro？arc oxidized titanium surfaces.