铁镍软磁合金细胞毒性研究

Research of the cytotoxicity of Fe-Ni soft magnetic alloy

目的：探讨铁镍软磁合金应用于临床的生物安全性。方法将5种材料（纯钛、铁镍合金、电镀Cr6＋的铁镍合金、钴铬合金、PVC）制成2 cm ×2 cm ×0.2 cm的方块，消毒灭菌后制取材料浸提液。取5块无菌96孔板，每孔加入100μl浓度为6×104个/ml对数生长期L？929细胞悬液，贴壁生长24 h后置换各组材料浸提液。培养1、2、3、4、5 d后各取出1块板，于倒置显微镜下观察各组细胞形态和贴壁情况，然后向每孔加入10μl CCK？8试剂，避光培养1.5 h后用酶标仪测定各孔吸光度（A）值。利用各组之间A值计算细胞相对增殖率（RGR），并对各组实验材料细胞毒性进行分级评价。结果在倒置显微镜下，除PVC出现大量细胞溶解、固缩外，其余各组细胞均形态正常、贴壁生长良好。根据各组材料细胞RGR进行毒性分级可知，除外PVC组为3级，其余各实验组材料毒性级别均为0~1级，可以应用于人体。结论铁镍合金原样和电镀Cr6＋后的细胞毒性均在安全范围。

Objective To research the cytotoxicity of Fe？Ni soft magnetic alloy in order to provide a biological basis for the clinical application. Methods Five groups of materials（Pure Ti,Fe？Ni alloy, Fe？Ni plated Cr6＋,Co？Cr alloy,and PVC）were cut into 2 cm × 2 cm × 0.2 cm blocks and prepared for the extract after sterilization. L？929 cells were incubated in five 96？well culture plates at a concentration of 6 × 103 per well for 24 h to allow the attachment. The culture medium was replaced with 100μl exaction per well. After 1,2,3,4 and 5 d culturing,the cells of each groups were observed under microscope. 10μl CCK？8 solution was added to each well and cultured for 1.5 h,the absorbance values were obtained with a spectrophotometer. The cytotoxicity of the materials was evaluated by calculating the relative growth rate （RGR）. Results The shape of L？929 cells of each group was normal besides the PVC group under the microscope. The cytotoxicity of PVC was grade 3,and all the other materials were grade 0~1 based on the RGR. All of the experimental materials were safe for clinical application except PVC. Conclusion Both Fe？Ni alloy and Fe？Ni plated Cr6＋have good biocompatibility.