诺氟沙星壳聚糖微球的制备及表征

Preparation and Characterization of NorfloxacinChitosan Microspheres

利用反相乳液聚合-化学交联法,以戊二醛作为交联剂制备诺氟沙星-壳聚糖微球.根据正交实验设计,考察核壳比、壳聚糖用量（w/v）、交联剂用量（v/v）和转速4 个因素对微球制备的影响.基于微球的载药量和包封率综合,得到最佳制备条件：（1）核壳比1：10,（2）壳聚糖用量（w/v） 4%,（3）交联剂用量（v/v）1%,（4）转速800 rpm.利用扫描电镜、红外光谱对最佳制备条件下得到的微球进行表征.测定壳聚糖微球在天然海水环境中48 h 内的释药速率,壳聚糖用量越大,药物释放越缓慢.

Norfloxacin （NFX）/ Chitosan （CS） microspheres were prepared by using inverse emulsion polymerization and cross-linkingmethod. Glutaraldehyde was used as cross linker. Orthogonal design based on 4 factors core-shell ratio, chitosan concentration,dosage of cross linker, agitation speed. Based on the entrapment efficiency and drug loading content of microspheres byorthogonal array analysis, the optimum condition for microspheres preparation was： core-shell ratio = 1：10, chitosan concentration（w/v） = 4%, dosage of cross linker （v/ v） = 1%, agitation speed = 800 rpm. The microspheres were characterized by Fouriertransform infrared spectroscopy （FTIR） and scanning electron microscopy （SEM）. Cumulative release profile of NFX from themicrospheres in seawater was measured in 48 h. The drug released slower as chitosan increases.