失血性休克后肠淋巴液引流恢复小鼠肾组织ACE/ACE2平衡的作用

Role of post-hemorrhagic shock mesenteric lymph drainage in restoring balance of ACE / ACE2 in kidney of mice

目的:观察失血性休克后肠淋巴液(PHSML)引流对失血性休克小鼠肾组织血管紧张素转换酶(ACE)/ACE2平衡的作用。方法:复制小鼠失血性休克模型,随机分为休克组与休克+引流组,行液体复苏;休克+引流组液体复苏后,引流肠淋巴液。在液体复苏后6 h,检测肾组织ACE、ACE2、血管紧张素II(Ang II)1型受体(AT1R)、Mas相关G蛋白偶联受体(MasR)的mRNA表达以及Ang II、Ang(1-7)含量。结果:失血性休克提高了肾组织ACE mRNA、AT1R mRNA和Ang II水平,降低了ACE2 mRNA、MasR mRNA和Ang(1-7)水平,PHSML引流抑制了失血性休克对ACE2和AT1R mRNA表达的影响;同时PHSML引流也降低了失血性休克增加ACE/ACE2、Ang II/Ang(1-7)和AT1R/MasR比值的作用。结论:PHSML引流恢复了失血性休克后肾组织的ACE/ACE2平衡,有利于减轻失血性休克所致的肾损伤。

AIM： To study the role of post-hemorrhagic shock mesenteric lymph （PHSML） drainage on the balance of angiotensin-converting enzyme （ACE） and ACE2 in the kidney. METHODS： A hemorrhagic shock model was established and then fluid resuscitation was performed to the animals in shock and shock ＋ drainage groups, and the PHMSL was drained in shock ＋ drainage group after fluid resuscitation. After 6 h of resuscitation, the mRNA expression of ACE, ACE2, angiotensin II （Ang II） type 1 receptor （ATIR） and Mas-related G-protein-coupled receptor （MasR）, and the levels of Ang II and Ang （ 1-7 ） in the renal tissues were observed. RESULTS ： Hemorrhagic shock increased the levels of ACE mRNA, ATI R mRNA and Ang II, and decreased the levels of ACE2 mRNA, MasR mRNA and Ang（1-7） in the kid- ney. PHSML drainage abolished the effect of hemorrhagic shock on ACE2 and AT1R mRNA expression. Meanwhile, PHSML drainage reduced the hemorrhagic shock-induced increases in the ratios of ACE/ACE2, Ang II/Ang （1-7） and AT1R/MasR. CONCLUSION ： The PHSML drainage restores the balance of ACE/ACE2, which is beneficial to alleviate acute kidney injury following hemorrhagic shock in the mice.