黄精多糖对小鼠心肌组织Ca^(2+)-ATP酶活性的影响

Influence of polygahatous polysaccharides on activity of Ca^(2+)-ATP enzyme in cardiac muscle tissue of mice

目的研究黄精多糖对小鼠心肌组织Ca泵(Ca2+-ATP酶)活性的影响。方法 72只昆明种小鼠随机分为对照组和黄精多糖低、剂量组、黄精多糖高剂量组,每组24只。经口灌胃4周,将三组小鼠分别于安静时、力竭即刻、力竭恢复24 h三种状态下断头处死,测定心肌组织中Ca2+-ATP酶的活性。结果力竭即刻及力竭恢复24 h,黄精多糖高剂量组Ca2+-ATP酶活性分别为(1.09±0.08)、(0.58±0.07),明显高于对照组和低剂量组(P<0.01)。结论黄精多糖可维持细胞膜内外Ca2+的分布平衡,效果以高剂量黄精多糖最佳。

Objective To research influence of polygahatous polysaccharides on activity of Ca prmp（Ca2＋-ATP enzyme） in cardiac muscle tissue of mice. Methods A total of 72 mice were randomly divided into control group, polygahatous polysaccharides low dose group and polygahatous polysaccharides high dose group, with 24 cases in each group. After lavage or 4 weeks, mice in the three groups were put to death at the time of rest state, at the immediate end of exhaustive exercise and in 24 hours after the end of exhaustive exercise, and their activity of Ca2＋-ATP enzyme in cardiac muscle tissue were detected. Results The high dose group had activity of Ca2＋-ATP enzyme at the immediate end of exhaustive exercise and in 24 hours after the end of exhaustive exercise as（1.09±0.08） and（0.58±0.07）. Those were much higher than the control group and low dose group（P0.01）. Conclusion Polygahatous polysaccharides can maintain the balance of Ca2＋ around cell membrane, and high dose can show precise effect.