乳腺纤维腺瘤雌激素受体与孕激素受体的表达分析

Expression analysis of breast ifbroadenoma of estrogen receptor and progesterone receptor

目的：研究雌激素受体（ER）、孕激素受体（PR）在乳腺纤维腺瘤组织及其周围正常组织中的表达状况，探讨其在乳腺纤维腺瘤发病中的作用。方法（1）标本来源：选择2013年1月至2014年8月厦门市妇幼保健院收治的经病理确诊为纤维腺瘤的患者447例，每例均切取肿瘤组织以及周围正常组织标本。（2）实验方法：ER、PR的表达状况采用免疫组织化学S-P 法分析，观察肿瘤组与正常组间ER、PR表达的差异；年龄对组织中ER、PR表达的影响。（3）统计学方法：采用SPSS 16.0统计软件进行数据分析。两组间ER、PR表达差异采用非参数的Wilcoxon检验，对于年龄与受体表达状况的相关性分析均采用Spearman相关性分析，以P〈0.05为差异有统计学意义。结果肿瘤组织ER、PR的表达明显高于正常组织，差异有统计学意义（P〈0.05）。在肿瘤组织中，年龄的变化与ER、PR的相关系数分别为-0.0314和0.0275（P〉0.05），差异无统计学意义；在正常组织中，年龄的变化与ER、PR的相关系数分别为-0.1226和-0.0860，年龄与ER的表达差异有统计学意义（P〈0.05），而与PR的表达虽然有负相关的趋势，但差异无统计学意义（P〉0.05）。结论乳腺纤维腺瘤腺上皮ER、PR的表达显著高于周围正常组织，表明激素受体的表达增高在乳腺纤维腺瘤的发病中起重要作用；纤维腺瘤激素受体的表达不像正常组织随着年龄增长而表达减弱，表明纤维腺瘤的激素受体高表达，有自主性。

Objective Study the expression of female hormonal receptors：estrogen receptors （ER）,progesterone receptor（PR） in breast fibroadenoma tissue and normal tissue around,exploring their role in the pathogenesis of breast fibroadenoma.Methods From January 2013 to August 2014,447 pairs paraffin slids were from the archived samples after operations and were pathological diagnosed with breast fibroadenomas in Xiamen Maternal and Child Health Hospital.The expressions of ER and PR were analysed with immunohistochemicaL（IHC） S-P method,each pair sample includes tumor tissue and normal tissue around.All the IHC results were analysed with SPSS 16.0 statistical software. ER,PR expression differences between the two groups using nonparametric Wilcoxon test inspection level （P〈0.05）;The correlation coefficient between age and hormonal receptors expression adopted Spearman correlation analysis, inspection level （P〈0.05）.Results The ER/PR expression of tumor group was obviously higher than that of normal tissue,The differences were statistically significant.The correlation coefficient of ER/PR expression between tumor tissues and age,no statistical significance.The correlation coefficient of ER expression in normal tissue and age is a statistical significance （P〈0.05）,with age increased, the ER expression decreased.Although a trend was observed that with the age increasing,the PR expression in normal tissue decreased.Conclusion Female breast fibroadenoma glandular epithelium average hormone receptors expression was significantly higher than that of the surrounding normal tissues,suggests that the high expression of hormone receptors in breast fibroadenoma,plays an important role in the pathogenesis and breast fibroadenoma tissue.Fibroadenoma hormone receptors,unlike normal tissue,with the increased age,the expression less,show that the high hormone receptors expression is autonomy.The exact process remains to be further researched.