摘要
目的：通过对ApoE基因敲除小鼠喂食搜风祛痰中药复方稳斑汤后观察动脉粥样硬化（AS）不稳定斑块自噬相关蛋白Beclin-1、Bcl-2、 LC3的改变，探索治疗动脉粥样硬化新思路、新方法。方法将ApoE基因敲除小鼠动脉粥样硬化不稳定斑块模型随机分成空白组、模型组、稳斑汤低、中、高剂量组、西药组。小鼠麻醉处死取主动脉组织HE染色后进行病理学观察，采用半定量 RT-PCR 技术检测Beclin-1、Bcl-2、LC3蛋白表达变化。结果与空白组比较，模型组小鼠腹主动脉组织中Beclin-1、Bcl-2、LC3蛋白表达水平显著降低（P〈0.01）；与模型组比较，西药组和稳斑汤各剂量组均能提高Beclin-1、Bcl-2、LC3表达水平（P〈0.01）；稳斑汤高剂量组与西药组在Beclin-1、Bcl-2、LC3表达水平上差异无统计学意义（P〉0.05）。结论中药搜风祛痰法稳斑汤可以显著提高Beclin-1、Bcl-2、LC3蛋白表达水平，从而达到稳定动脉硬化斑块、抗动脉硬化效果。
ObjectiveTo observe the effects of Soufengqutan Chinese herbal compound Wenban Decoction on ApoE knockout mice AS unstable plaque animal models.Methods Tobuild the ApoE knockout mice AS unstable plaque animal models and divide them into blank control group,model control group,Western medicine group and Wenban Decoction low,middle and high dose group.After the implementation of anesthesia and execution,take out the aorta tissue in mice which should be HE stained and then pathologically observed under the optical microscope.Then detect the expression changes of Beclin-1 Bcl-2 and LC3 with semi- quantitative RT-PCR technique.Results Beclin-1 LC3 expression levels in the model group were significantly lower than those of normal group（P〈0.01）.Bcl-2 expression levels in the model group were significantly lower than those of normal group（P〈0.05）.Compared with model group,Beclin-1 Bcl-2 and LC3 expression levels in each medicated group were significantly higher （P〈0.01）.There was no difference between Western medicine group and Wenban Decoction high dose group in expression levels of Beclin-1 Bcl-2 and LC3 （P〉0.05）.Conclution Soufengqutan Chinese herbal compound Wenban Decoction may intervene the formation and eruption of AS unstable plaque through up-regulating the expression of Beclin-1 Bcl-2 and LC3 in ApoE knockout mice AS unstable plaque,which makes the cardiovascular system well protected.
出处
《中国临床实用医学》
2015年第5期37-40,共4页
China Clinical Practical Medicine
基金
中国博士后基金面上项目（20110491540）
辽宁省百千万人才项目（2011921022）
辽宁省自然基金项目（2014020055）
