hTERT和E2F-1蛋白在结直肠癌组织中的表达及其临床意义

Expressions of h TERT and E2F-1 proteins in colorectal carcinoma tissues and their clinical significance

目的 :探讨人端粒酶逆转录酶(human telomerase reverse transcriptase,h TERT)和转录因子E2F-1在结直肠癌组织中的表达及其临床意义。方法 :应用免疫组织化学法检测134例结直肠癌、74例结直肠腺瘤和28例正常结直肠黏膜组织中h TERT和E2F-1蛋白的表达,分析h TERT和E2F-1蛋白的表达与结直肠癌患者临床病理特征及预后之间的关系。结果 :h TERT蛋白在结直肠癌、结直肠腺瘤和正常结直肠黏膜组织中的阳性表达率分别为47.0%、40.5%和28.6%(P>0.05),h TERT在结直肠癌组织中的表达与Dukes分期和淋巴结转移有关(P<0.05)。E2F-1蛋白在结直肠癌、结直肠腺瘤和正常结直肠黏膜组织中的阳性表达率分别为72.4%、95.9%和100.0%(P<0.05),E2F-1在结直肠癌组织中的表达与Dukes分期和淋巴结转移有关(P<0.05)。h TERT阳性组中,Dukes C+D期患者化疗后生存率明显提高(P<0.05)。Dukes分期和h TERT蛋白表达是影响结直肠癌患者的独立预后因素(P<0.05)。结论 :h TERT和E2F-1蛋白表达与结直肠癌患者的疾病进展及预后有关,E2F-1可能参与了结直肠癌的发生,Dukes C+D期结直肠癌患者进行h TERT检测对手术后化疗有一定的指导意义。

Objective： To investigate the expressions of human telomerase reverse transcriptase （hTERT） and E2F-1 proteins in colorectal carcinoma tissues and their clinical significance. Methods： The expressions of hTERT and E2F-1 specimens of colorectal carcinoma tissues, 74 speci adenoma tissues and 28 specimens of normal c proteins in 134 mens of colorectal olo tissues were detected by immunohistochemistry. The expressions of hTERT and E2F-1 proteins with clinica rectal mucosa correlations of characteristics and prognosis of patients with colorectal carcinoma were analyzed Results： The positive rates of hTERT protein expression in colorectal carcinoma, colorectal adenoma and normal colorectal mucosa tissues were 47.0%, 40.5% and 28.6%, respectively （P 〉 0.05）. The expression of hTERT in colorectal carcinoma tissues was correlated with Dukes stage and lymphatic metastasis （both P 〈 0.05）. The positive rates of E2F-1 protein expression in colorectal carcinoma, colorectal adenoma and normal colorectal mucosa tissues were 72.4%, 95.9% and 100.0%, respectively （P 〈 0.05）. The expression of E2F-1 protein in colorectal carcinoma tissues was correlated with Dukes stage and lymphatic metastasis （both P 〈 0.05）. For colorectal cancer patients with positive expression of hTERT protein, the survival of the patients with Dukes stages C and D after chemotherapy was improved （P 〈 0.05）. Dukes stage and the expression of hTERT protein were independent prognostic factors for colorectal cancer patients （both P 〈 0.05）. Conclusion： The expressions of hTERT and E2F-1 proteins are closely related to the progression and prognosis of patiens with colorectal carcinoma. E2F-1 may play a role in tumorigenesis of colorectal cancer. Detection of hTERT protein may be helpful to determine post-operative chemotherapy for patients with Dukes stages C and D colorectal carcinoma.