AM真菌对滇重楼光合参数及生理指标的影响

Photosynthetic parameters and physiological indexes of Paris polyphylla var. yunnanensis influenced by arbuscular mycorrhizal fungi

通过室温盆栽接种试验与室内分析相结合的方法,观察灭菌土壤条件下接种28种丛枝菌根(AM)真菌对滇重楼光合参数和生理生化指标的影响。结果表明,滇重楼与AM真菌形成了良好的共生关系。AM真菌诱导处理后,不同AM真菌对滇重楼叶片的光合参数和生理生化指标的影响有所差异,AM真菌的施用能够提高滇重楼叶肉细胞的光合作用强度,提高滇重楼叶片可溶性蛋白和可溶性糖含量及保护酶活性,增强了滇重楼抗不利环境的能力,促进了滇重楼的生长,而滇重楼与AM真菌之间存在一定的相互选择性。从综合接种效应来看,瑚状盾巨囊霉Racocetra coralloidea、美丽盾巨孢囊霉Scutellospora calospora、近明球囊霉Claroideoglomus claroideum、透明盾巨孢囊霉S.pellucida和明球囊霉Rhizophagus clarus是最适宜滇重楼大田栽培时推广应用的优良备选菌株。

Through potted inoculation test at room temperature and indoor analysis,the photosynthetic parameters and physiological and biochemical indexes of Paris polyphylla var. yunnanensis were observed after 28 arbuscular mycorrhizal（ AM） fungi were injected into the P. polyphylla var. yunnanensis growing in a sterile soil environment. The results showed that AM fungi established a good symbiosis with P. polyphylla var. yunnanensis. The AM fungi influenced the photosynthetic parameters and physiological and biochemical indexes of P. polyphylla var. yunnanensis. And the influences were varied depending on different AM fungi. The application of AM fungi improved photosynthesis intensity of P. polyphylla var. yunnanensis mesophyll cells,the contents of soluble protein and soluble sugar,protective enzyme activity of P. polyphylla var. yunnanensis leaf,which was beneficial to resist the adverse environment and promote the growth of P. polyphylla var. yunnanensis. Otherwise,there was a certain mutual selectivity between P. polyphylla var.yunnanensis and AM fungi. From the comprehensive effect of inoculation,Racocetra coralloidea,Scutellospora calospora,Claroideoglomus claroideum,S. pellucida and Rhizophagus clarus were the most suitable AM fungi to P. polyphylla var. yunnanensis when P.polyphylla var. yunnanensis was planted in the field.