白头翁皂苷对佐剂性关节炎大鼠FZD8表达的影响

Effect of pulchinenoside on FZD8 expression of adjuvant arthritis rats

研究中药白头翁有效成分白头翁皂苷(pulchinenoside,PULC)对佐剂性关节炎(adjuvant arthritis,AA)大鼠Frizzled(FZD)表达的影响。采用足跖注射完全弗氏佐剂制备AA大鼠,原代培养AA大鼠滑膜成纤维样滑膜细胞(fibroblast-like synoviocytes,FLS),Real-time q PCR检测PULC灌胃治疗对AA大鼠FLS FZD8表达的影响,MTT,ELISA检测FZD8敲除对FLS增殖和IL-1,IL-6,IL-8表达的影响,Real-time q PCR检测miR-375在FZD8异常表达中的作用。检测发现经PULC灌胃治疗后,AA大鼠FLS中FZD8表达明显降低,FZD8敲除后FLS增殖明显减弱,IL-1,IL-6,IL-8表达明显减少,PULC灌胃治疗后miR-375表达明显升高,上调表达的miR-375能够抑制FZD8的表达。PULC可能通过上调miR-375表达抑制FZD8的表达。

To study the effect of pulchinenoside（ PULC） on the Frizzled（ FZD） expression of adjuvant arthritis（ AA） rats. AA rats were prepared through the toe injection with complete Freund＇s adjuvant to culture fibroblast-like synoviocytes（ FLS）. The effect of the oral administration with PULC on the FZD8 expression was detected by the real time q PCR. The effect of FZD8 knockout on the expressions of IL-1,IL-6,IL-8 were detected by MTT and ELISA. The role of miR-375 in the abnomal expression of FZD8 was detected by the real time q PCR. The results showed signfiicant decrease in the FZD8 expression among AA rats,FLS proliferation ater FZD8 knockout and IL-1,IL-6,IL-8 expressions and notable increase in miR-375 expression after the oral administration with PULC. The up-regulated miR-375 expression can inhibit the FZD8 expression. PULC may inhibit the FZD8 expression by up-regulating the miR-375 expression.