apoAV-1131 T>C基因多态性与非酒精性脂肪性肝病的关系研究

Research on the relationship between of the apolipoprotein AV and non-alcoholic fatty liver disease

[目的]了解载脂蛋白AV(apoAV)-1131 T>C基因多态性和apoAV水平与非酒精性脂肪性肝病(NAFLD)的关系。[方法]对78例B超确诊为NAFLD患者(NAFLD组)和58例同期体检示非NAFLD者(对照组),采用酶联免疫吸附试验检测血清apoAV浓度进行测定,通过聚合酶联反应-限制性长度多态性方法进行apoAV-1131 T>C基因多态性分析。[结果]NAFLD组的apoAV水平低于对照组、TC基因型频率高于对照组(均P<0.05);TC+CC基因型的血清apoAV水平低于TT基因型、血清三酰甘油水平高于TT基因型(均P<0.05)。[结论]apoAV-1131 T>C位点影响血清apoAV和三酰甘油水平,增加了NAFLD的发病风险。

[Objective]To detect the apolipoprotein AV（apoAV）‐1131 T〉C gene polymorphism and se‐rum apoAV level in non‐alcoholic fatty liver disease（NAFLD）patients and explore the relationship.[Meth‐ods]Serum apoAV concentration in seventy‐eight cases of NAFLD diagnosed by ultrasound and fifty‐eight cases of normal control was measured by using enzyme‐linked immunosorbent assay.The apolipoprotein AV‐1131 T〉C gene polymorphism was detected by the polymerase reaction‐limiting length polymorphism methods.[Results]ApoAV level of NAFLD group was obviously lower than normal group.TC genotype frequency in NAFLD group was significantly higher than normal group（P〈 0.05）.TC/CC genotype of se‐rum apoAV was significantly lower than TT genotype ,TC/CC genotype of serum triglycerides level was higher than T T genotype.[Conclusion]apoAV‐1131 T〉C sites affect serum apoAV and TG levels ,and in‐crease the risk of NAFLD.