摘要
目的探讨肥大细胞改变炎症性肠病(inflammatory bowel disease,IBD)动物模型中结肠黏膜紧密连接蛋白-(Claudin-3)表达及其通透性的研究。方法 60只3个月小鼠分为三组:白介素-10(IL-10)基因缺陷组(IL-10-/-)、IL-10基因缺陷及肥大细胞基因部分缺陷组(IL-10-/-/Sash+/-)和IL-10基因缺陷及肥大细胞基因缺陷组(IL-10-/-/Sash-/-,DKO)。采用Ussing chamber检测结肠上皮通透性;HE染色评价各组结肠损伤严重程度;免疫组化评估结肠claudin-3表达。结果IL-10-/-/Sash+/-组、DKO组结肠黏膜损伤较IL-10-/-组明显严重(P<0.05)。与IL-10-/-组比,IL-10-/-/Sash+/-组、DKO组肠上皮跨膜电阻抗值和跨膜电势差显著降低(P<0.05),短路电流值明显增加(P<0.05)。三组的结肠均表达claudin-3,IL-10-/-/Sash+/-组、DKO组的claudin-3表达较IL-10-/-组明显降低(P<0.05)。结论肥大细胞通过上调结肠Claudin-3的表达而达到保护肠黏膜屏障功能的作用。
Objective To investigate the role of mast cells in claudin-3expression and permeability of colon mucosa in animal model of inflammatory bowel disease.Method Mice of 3months were divided into 3groups which were IL-10-deficient group（IL-10-/-）,IL-10-deficient with half mast cells group（IL-10-/-/Sash＋/-）,and IL-10-deficient without mast cells group（IL-10-/-/Sash-/-,DKO）.Histopathological scores of colon samples were tested by HE staining.Immunohistochemistry was used to measure the expression of claudin-3in colon tissue.The transepithelial electric resistance（TEER）,transepithelial potential difference（TEPD）and short circuit current（Isc）of the specimens were measured by using chamber.Result The degree of histopathological scores in IL-10-/-group was significantly lower than that in IL-10-/-/Sash＋/-and DKO groups（P〈0.05）.IL-10-/-/Sash＋/-and DKO groups showed significantly decreased TEER（P〈0.05）and TEPD（P〈0.05）,and significantly increased Isc（P〈0.05）compared with IL-10-/-group.The expression levels of claudin 3in IL-10-/-/Sash＋/-and DKO groups were significantly decreased compared with that in IL-10-/-group（P〈0.05）.Conclusion Mast cells appear to enhance the efficacy of barrier function colon mucosa by increasing the expression of claudin 3.
出处
《贵州医药》
CAS
2015年第7期589-591,F0003,共4页
Guizhou Medical Journal
基金
深圳市科技项目计划(201003242)
