hNIS基因转染SW480细胞裸鼠模型的建立及核素显像

The construction of SW480 cells nude model transfected by h NIS gene and radionuclide imaging

目的:观察人钠/碘转运体(h NIS)重组质粒(pc DNA3.1+-h NIS)脂质体法转染结肠癌细胞(SW480)的裸鼠模型介导放射性核素显像的可行性。方法:酶切鉴定pc DNA3.1+-h NIS,转染SW480细胞并检测h NIS基因及蛋白的表达,筛选稳定表达h NIS的SW480细胞,建立肿瘤裸鼠模型并进行放射性核素99mTc O4-显像。结果:pc DNA3.1+-h NIS转染SW480细胞后成功检测到h NIS基因及蛋白的表达,结肠癌裸鼠模型成功介导放射性核素99mTc O4-显像。结论:重组质粒pc DNA3.1+-h NIS可以实现结肠癌裸鼠模型的h NIS表达,使放射性核素显像和结肠癌放射性核素治疗成为可能。

Objective： To examine the feasibity of radionuclide imaging mediated by colon cancer cells （SW480） nude model transfected with a recombinant pcDNA3. I＋-hNIS plasmid. Methods： pcDNA3.1＋-hNIS was confirmed by restriction enzyme digestion, the hNIS gene and protein expression were detected after trans- fection, the SW480 cells of stably expressing hNIS were screened, the tumors nude mice model was established, then radioactive nuclide 99mTcO4- imaging was obtained. Results： After SW480 cells was transfected with pcDNA3. 1＋-hNIS, the hNIS gene and prote in expression were successfully detected. Radionuclide imaging was successfully mediated by colon cancer cells nude model. Conclusion： The hNIS expression can be achieved in colon nude model by the recombinant plasmid pcDNA3. 1＋-hNIS, and make the radionuclide imaging and radio- nuclide therapy of colon cancer as possible.