HPLC法测定中药愈伤便捷湿巾药液中栀子苷的含量

Content Determination of Geniposide in TCM Callus Convenient Wipes Drug Solution by HPLC

目的：建立测定中药愈伤便捷湿巾药液中栀子苷含量的方法。方法：采用高效液相色谱法。色谱柱为Aglient Zorbax SB-C18,流动相为乙腈-0.1%磷酸（10∶90,V/V）,流速为1.0 ml/min,检测波长为236 nm,柱温为30℃,进样量为10μl。结果：栀子苷的检测进样量线性范围为0.104 1~1.041μg（r=0.999 8）;精密度、稳定性、重复性试验的RSD均〈2%;加样回收率为99.04%~100.82%,RSD为0.85%（n=6）。结论：该方法简便、准确、重复性好,可用于中药愈伤便捷湿巾药液中栀子苷的含量测定。

OBJECTIVE：To establish a method for the content determination of geniposide in TCM callus convenient wipes drug solution. METHODS：HPLC was performed on the column of Agilent Zorbax SB-C18 with mobile phase of acetonitrile-0.1%phosphoric（10∶90,V/V）at the flow rate of 1.0 ml/min,column temperature was 30 ℃,detection wavelength was 236 nm and the volume was 10 μl. RESULTS：The linear range of geniposide was 0.104 1-1.041 μg（r=0.999 8）;RSDs of precision,stability and reproducibility tests were all lower than 2%;recovery was 99.04%-100.82%（RSD=0.85%,n=6）. CONCLUSIONS：The method is simple,accurate,reproducible,and can be used for the content determination of geniposide in TCM callus convenient wipes drug solution.