HPLC法测定余甘子不同提取部位中没食子酸的含量

Content Determination of Gallic Acid in Different Preparation Parts of Phyllanthus emblica by HPLC

目的：建立测定余甘子不同提取部位中没食子酸含量的方法。方法：采用高效液相色谱法。色谱柱为ZORBAX Extend C18,流动相为甲醇~0.1%磷酸（10∶90,V/V）,流速为1.0 ml/min,检测波长为270 nm,柱温为30℃,进样量为10μl。结果：没食子酸检测质量浓度线性范围为0.042 5~0.212 5 mg/ml;精密度、稳定性、重复性试验的RSD〈3.0%;加样回收率为99.38%~102.14%（RSD=1.045,n=6）。余甘子中没食子酸为1.82%,各不同制备部位没食子酸含量为0.70%~2.38%。结论：该方法操作简便、重复性好,可用于余甘子不同提取部位中没食子酸含量的测定。

OBJECTIVE：To establish a method for the content determination of gallic acid in different preparation parts of Phyllanthus emblica. METHODS：HPLC was performed on the column of ZORBAX Extend C18 with mobile phase of methanol-0.1%phosphoric acid（10∶90,V/V）at flow rate of 1.0 ml/min,detection wavelength was 270 nm,column temperature was 30 ℃ and the volume injection was 10 μl. RESULTS：The linear range of gallic acid was 0.042 5-0.212 5 mg/ml;RSDs of precision,accuracy and stability tests were lower than 3.0%;recovery was 99.38%-102.14%（RSD=1.045,n=6）. The mass fraction of gallic acid in P. emblica was 1.80%,and the content of gallic acid in different preparation parts was 0.70%-2.38%. CONCLUSIONS：The method is simple,reproducibility,and can be used for the content determination of gallic acid in different preparation parts of P. emblica.