摘要
基于没食子酸丙酯(PG)对Fenton试剂氧化固绿褪色反应具有明显抑制作用的原理,建立了一种用分光光度法测定食品中PG新方法。研究了固绿用量等因素对体系的影响,优化了实验条件。在选定的实验条件下,方法的线性回归方程为ΔA_(625nm)=59.328ρ+0.008 5,相关系数(R^2)为0.995 2,线性范围为8.3×10^(-4)~1.6×10^(-4)mg/mL,检出限为9.8×10^(-5)mg/mL。可用于测定食品中的PG,测定结果的RSD为3.4%,回收率在96.25%~103.3%。
Based on gallic acid ester (PG) of Fenton reagent oxidation solid green fade has obvious inhibitory effect principle, A new method of determination of PG in food was established using speetrophotometric method. The influence of the solid green consumption and other factors on the system were studied, the experimental conditions were optimized. In selected experimental conditions, the method of linear regression equation for delta rho △A625nm = 59.328p + 0.008 5, the correlation coefficient ( R2 ) of 0.995 2, the linear range of 8.3 × 10-4 - 1.6 ×10-2 mg/mL, detection limit of 9.8 × 10-5 mg/mL. Method used in the determination of food in PG, the determination results of RSD was 3.4%, the recovery in 96.25% - 103.3%.
出处
《粮油食品科技》
2015年第6期92-95,共4页
Science and Technology of Cereals,Oils and Foods
基金
桂林区域生态环境分析和污染控制重点实验室资助项目
广西教育厅高校科学技术研究项目(LX2014441)