固绿褪色光度法测定食品中痕量没食子酸丙酯

Fading spectrophotometric method for the determination of trace propyl gallate in food with FCF-fenton system

基于没食子酸丙酯(PG)对Fenton试剂氧化固绿褪色反应具有明显抑制作用的原理,建立了一种用分光光度法测定食品中PG新方法。研究了固绿用量等因素对体系的影响,优化了实验条件。在选定的实验条件下,方法的线性回归方程为ΔA_(625nm)=59.328ρ+0.008 5,相关系数(R^2)为0.995 2,线性范围为8.3×10^(-4)~1.6×10^(-4)mg/mL,检出限为9.8×10^(-5)mg/mL。可用于测定食品中的PG,测定结果的RSD为3.4%,回收率在96.25%~103.3%。

Based on gallic acid ester （PG） of Fenton reagent oxidation solid green fade has obvious inhibitory effect principle, A new method of determination of PG in food was established using speetrophotometric method. The influence of the solid green consumption and other factors on the system were studied, the experimental conditions were optimized. In selected experimental conditions, the method of linear regression equation for delta rho △A625nm = 59.328p ＋ 0.008 5, the correlation coefficient （ R2 ） of 0.995 2, the linear range of 8.3 × 10-4 - 1.6 ×10-2 mg/mL, detection limit of 9.8 × 10-5 mg/mL. Method used in the determination of food in PG, the determination results of RSD was 3.4%, the recovery in 96.25% - 103.3%.