受体酪氨酸激酶EphA4蛋白在乳腺癌组织中的表达及其临床意义

Expression of receptor tyrosine kinase Eph A4 protein in breast carcinoma and its clinical significance in breast carcinoma

目的探讨Eph A4蛋白在乳腺癌组织中的表达及其与临床病理特征之间的关系。方法收集未经治疗的乳腺浸润性导管癌手术标本77例,用Eph A4抗体进行免疫组化En Vision法染色,分析乳腺癌细胞染色强度与临床病理特征的相关性。结果 Eph A4在正常乳腺细胞中66.2%呈强阳性(■),19.5%呈中度阳性(■),14.3%呈弱阳性(-^+)。Eph A4蛋白在浸润性导管癌中20.8%呈强阳性(■),24.7%呈中度阳性(■),54.5%呈弱阳性(-^+)。Eph A4蛋白在正常乳腺细胞和浸润性导管癌细胞之间的表达差异有显著性(P<0.001)。Eph A4蛋白表达丢失与肿瘤高分级(P=0.029)、TNM病理分期(P=0.034)和Ki-67高增殖指数(P<0.001)呈正相关;与患者年龄(P=0.940)、淋巴结转移(P=0.329)、分子分型(P=0.945)和HER-2表达(P=0.431)无相关性。结论 Eph A4蛋白在浸润性导管癌中表达下调,提示Eph A4基因在乳腺癌中具有抑癌的作用,是潜在的诊断和治疗新靶标。

Purpose To explored the expression of EphA4 protein in breast carcinoma and to study the relationship between expression and clinicopathologic parameters. Methods 77 cases of invasive ductal carcinoma without chemotherapy were collected. The EphA4 protein was detected by immunohistochemistry method. The relationship between EphA4 expression score and clinicopathologic parameters was analyzed. Results EphA4 protein was positively expressed in normal breast luminal cells, of which 66. 2% were score （ ） , 19. 5% were score （ ） and 14. 3% were score （ - - ＋ ）. EphA4 protein was down-regulated in breast carcinoma cells, of which 20. 8% were score （ ）, 24. 7% were score （ ） and 54. 5% were score （ - - ＋ ）. The significant difference in expression of EphA4 was observed between normal breast and carcinomas （ P 〈 0. 001 ）. Loss of EphA4 protein was positively related to grade （ P = 0. 029 ） , TNM stage （ P = 0. 034） and Ki-67 expression （ P 〈 0. 001 ）. No significance was observed between EphA4 expression and age （ P = 0. 940）, lymph node metastasis （ P = 0. 329）, molecular classification （ P = 0. 945 ） and HER-2 expression （ P = 0. 431 ）. Conclusion EphA4 protein was down-regulated in breast invasive ductal carcinoma. EphA4 plays a tumor inhibitor in breast carcinoma and may be a potential target for diagnosis and therapy.