微小RNA-155对胃癌细胞株SGC7901生物学行为的影响

Effect of microRNA-155 on the biological behaviors of gastric carcinoma SGC7901 cells

目的 观察微小RNA-155（miR-155）对胃癌细胞增殖、凋亡、侵袭及迁移的影响.方法 实验分未转染组、空白对照组、miR-155模拟物转染组及miR-155抑制物转染组4组,分别将miR-155模拟物及抑制物瞬时转染胃癌细胞株SGC7901,实时定量反转录聚合酶链反应（RT-qPCR）检测转染细胞的miR-155表达水平,细胞计数试剂盒（CCK-8法）检测细胞增殖能力,流式细胞术检测细胞凋亡及细胞周期,Transwell小室检测细胞体外侵袭迁移能力.结果 RT-qPCR显示：转染miR-155模拟物后,SGC7901细胞miR-155表达水平明显上调,而转染miR-155抑制物后表达明显受抑（P＜0.01）.miR-155模拟物转染组细胞增殖活性、迁移和侵袭能力明显增强（P＜0.05）,凋亡率（1.68±0.34）％较未转染组（4.23±0.52）％、空白对照组（3.43±0.61）％和抑制物转染组（9.10±2.13）％明显降低（P＜0.05）.转染miR-155抑制物后,出现G0/G1期阻滞,G0/G1期细胞增多达（70.63±1.12）％.结论 miR-155能促进胃癌细胞株SGC7901增殖,抑制其凋亡,增强其迁移及侵袭能力.

Objective To explore the effect of microRNA-155 （miR-155） on the biological behaviors of human gastric carcinoma cells.Methods Experiments were divided into four groups ： negative control group and blank control group, miR-155 mimics group, and miR-155 inhibitor group.miR-155 mimics group was transfected with miR-155 mimics to enhance the functions of miR-155, and miR-155 inhibitor group was transfected with miR-155 inhibitor.miR-155 mRNA expression was detected by real-time quantitative reverse transcriptase-polymerase chain reaction （RT-qPCR）.Cell proliferation was evaluated using the cell counting kit-8 （CCK-8） assay and apoptosis was assessed by flow cytometry.Invasion and migration were measured by Transwell chamber assays.Results The SGC7901 cells transfected with miR-155 mimics showed significantly higher miR-155 mRNA expression than the non-transfected SGC7901 cells and the transfected control cells （P 〈 0.01）.The miR-155 mRNA expression was significantly lower in the SGC7901 cells transfected with the miR-155 inhibitor than the non-transfected SGC7901 cells and the transfected control cells （P 〈 0.01）.The overexpression of miR-155 promoted the viability, and invasion and migration abilities, as shown in the cells transfected with the miR-155 mimics （P 〈 0.05）.There was no significant difference between control group and blank group, while the apoptosis rate in the mimics group [（1.68 ± 0.34） %] was lower than that in the control group [（4.23 ± 0.52） %], blank group [（3.43 ± 0.61） %] and inhibitor group [（9.10-± 2.13）%] （P〈0.05）, and the cell cycle was significantly arrested in G0/G1 phase and the number of G0/G1 phase cells was increased larger than （70.63 ± 1.12） % by flow cytometry.Conclusion miR-155 had a promotable effect on cell proliferation, inhibited apoptosis, and markedly promoted invasion and migration of SGC7901 cells.