氢气盐溶液对大鼠脑缺血再灌注后海马区损伤的保护作用及其机制

Preventive effect of hydrogen-rich saline on oxidative stress of ischemia reperfusion in rat hippocampi

目的 探讨氢气盐溶液对大鼠脑缺血-再灌注损伤的保护作用及机制.方法 将SD大鼠90只随机分为3组（n=30）：对照组（C组）,大鼠行双侧颈总动脉游离不进行夹闭,腹腔注射4℃生理盐水5 ml/kg.生理盐水组（S组）,大鼠行双侧颈总动脉游离后,结扎双侧颈总动脉15 min,腹腔注射生理盐水5 ml/kg.氢气盐溶液组（H组）：大鼠行双侧颈总动脉游离后,结扎双侧颈总动脉15 min,腹腔注射氢气盐溶液5 ml/kg.处理后24、48、72 h,测定大鼠脑组织中丙二醛（MDA）含量,总超氧化物歧化酶（T-SOD）与谷胱甘肽过氧化物酶（GSH-Px）活性,脑组织海马区切片尼氏体染色,观察海马区损伤.处理后第3天每组的10只大鼠在取脑组织标本前,通过水迷宫测试各组大鼠认知功能.结果 与S组比较,H组缺血再灌注后,脑组织中MDA含量升高程度明显降低（24、48、72 h分别为6.23±0.60比4.45±0.57,6.40±0.59比4.37±0.70,6.37 ±0.49比4.21 ±0.62,P＜0.05）,T-SOD活性明显升高（24、48、72 h分别为115.35 ±8.63比137.16 ±6.91,116.67±7.68比140.21 ±5.63,106.32±6.94比136.56±7.28,P＜0.05）,和GSH-Px活性明显升高（24、48、72 h分别为3.35±0.34比3.98±0.25,2.91±0.16比4.62±0.31,3.01 ±0.23比4.79±0.15,P＜0.05）.同时大鼠的海马区细胞受损明显减轻,水迷宫实验中大鼠第1次登台的时间及路程明显缩短（21.36±7.59比17.39 ±7.63,58.73±19.32比33.67±19.32,P＜0.05）.结论 氢气盐溶液腹腔内注射能够减轻大鼠脑缺血再灌注导致的海马区损伤.

Objective To investigate the protective effects of hydrogen-rich saline on ischemia/ reperfusion injury of brain in rats and the mechanism.Methods 90 SD rats were divided into three groups randomly.In control group （C, n =30）, bilateral common carotid artery （CCA） was separated, and 4 ℃ normal saline solution （5 ml/kg） was injected intraperitoneally.In saline group （S, n =30）, bilateral CCA was occluded for 15 min and 4℃ normal saline （5 ml/kg） was injected intraperitoneally.In hydrogen-rich saline group （H, n =30）, bilateral CCA was occluded for 15 min and 4℃ hydrogen-rich saline was injected intraperitoneally.Malonaldehyde （MDA）, glutathione peroxidase （GSH-Px） and total superoxide dismutase （T-SOD） were measured at 24, 48, and 72 h after operation.At the same time the sections of the hippocampi were dyed.Morris water maze test was developed at 3rd day after operation.Results At 24, 48 and 72 h, as compared with S group, the contents of MDA were significantly reduced 6.23 ± 0.60 vs.4.45 ± 0.57, 6.40 ± 0.59 vs.4.37 ± 0.70, and 6.37 ± 0.49 vs.4.21 ± 0.62, P 〈 0.05）, the activity of GSH-Px was significantly increased （3.35 ± 0.34 vs.3.98 ± 0.25, 2.91 ± 0.16 vs.4.62 ± 0.31, and 3.01 ±0.23 vs.4.79 ± 0.15, P 〈 0.05）, and the activity of T-SOD was significantly increased also （115.35 ±8.63 vs.137.16±6.91, 116.67 ±7.68 vs.140.21 ±5.63, and 106.32 ±6.94 vs.136.56± 7.28 （P 〈 0.05 for all） in H group.The injury of the hippocampi was significantly alleviated in H group as compared with S group.The time of the first stage and distance in morris water maze test were significantly shortened in H group H as compared with S group.Conclusion Our data demonstrate that intraperitoneal injection of hydrogen-rich saline has strong protective effect on the oxidative stress of ischemia reperfusion in rat brain.