HS-SPME-GC-MS分析普洱茶中木香类特征香气成分

Analysis of Wood Aroma Characteristic Compounds in Pu-erh Tea by HS-SPME-GC-MS

利用响应面法对HS-SPME萃取普洱茶木香化合物的条件进行优化。在单因素实验基础上,采用Central-Composite试验设计,以装茶量、萃取时间和萃取温度为影响因素,以木香化合物总峰面积为响应值建立二次回归方程。通过对各因素的显著性和交互作用进行分析后,结合实际情况得到木香化合物最佳萃取条件为:装茶量为3.5g,萃取时间为56min,萃取温度为61℃。此条件下,SPME萃取的木香化合物经GC-MS测得总峰面积为159284923,与预测值一致,说明模型准确性较高,随后对5种普洱茶茶样的木香化合物进行了萃取分析。

The response surface method was used to optimize headspace solid-phase microextraction （HS-SPME） conditio in extracting woody aroma compounds of pu-erh tea. Based on single factor experiment,taking amount of pu-erh tea, extraction time and extraction temperature as the influence factor,a quadratic regressive equation based on response value of total peak area of woody aroma compounds from pu-erh teawas established. Subsequently,response surface methodology based on Central-Composite experimental design was employed to evaluate the significance and interactive effects of those three variables. The results showed that the optimal extraction conditions of woody aroma compounds were as follows： the amount of pu-erh tea 3.5 g,extraction time 56min and extraction temperature 61℃. Under the optimal conditions,thetotal peak area of woody aroma compounds was up to 159284923,which was in agreement with the calculated value and indicated that the model accuracy is high. Then,the woody aroma compounds from5 pu-erh tea samples were extracted and detected.