纳米胶体钯-石墨炉原子吸收光谱法快速测定血中铅、镉

Rapid determination of lead,cadmium in blood by graphite furnace atomic absorption spectrometry with nanometer colloid palladium

目的探讨纳米胶体钯在石墨炉原子吸收光谱法测定血样铅、镉中的应用,建立快速、稳定、灵敏、准确的测定方法。方法血样经稀释后,以纳米胶体钯为基体改进剂,用石墨炉原子吸收光谱法测定铅、镉的浓度。结果本法在铅浓度为0μg/L^40.0μg/L、镉浓度为0μg/L^3.0μg/L的线性相关系数均>0.999,铅的检出限为3.22μg/L,回收率为90.1%~106.2%;镉的检出限为0.28μg/L,回收率为90.6%~103.4%,相对标准偏差均<10%,同时对GBW09139和GBW09140冻干牛血国家标准物质进行测定,结果均在标准值范围内。结论该法简便快速、灵敏度高、重现性好,适用于人血液中微量铅、镉的快速批量测定。

Objective To establish a fast,stable sensitive and precise method for determination of lead and cadmium in blood by graphite furnace atomic absorption spectrometry（ GF- AAS） with nanometer colloid palladium. Methods After the blood sample was dilute with nanometer colloid palladium as matrix modifier,the content of lead and cadmium were determined by graphite furnace atomic absorption spectrometry. Results Within the rang of lead 0 μg / L to 40. 0 μg / L,and cadimium 0 μg / L to 3. 0 μg / L,the linear correation coefficients were greater than 0. 999. The detection limit of lead was 3. 22 μg / L and the recovery rates were 90. 1%- 106. 2%. The detection limit of cadmium was 0. 28 μg / L,and the recovery rates were 90. 6%-103. 4%. The relative standard deviations for lead and cadmium were all less than 10%. The freeze dried cow blood in national standards substance（ GBW09139 and GBW09140） have been determined and the results were within the range of the given value. Conclu sion The method is simple,rapid,sensitive,reproducible,and it is suitable for batch determination of trace lead,cadmium in human blood.