参加抗病毒治疗的艾滋病患者的HIV-1基因亚型分布

Distribution of HIV-1 gene subtypes in AIDS patients with anti-virus treatment

目的分析参加抗病毒治疗的艾滋病患者的HIV-1基因亚型分布情况。方法采用巢式PCR法扩增pol区基因,测定蛋白酶编码区和逆转酶编码区的基因序列,并与国际标准亚型毒株进行对比。使用Mega 5.0软件分别构建测得序列与国际参考序列的蛋白酶编码区和逆转录酶编码区N-J系统发育树,分析基因亚型。结果 55份样本中有48份样本的HIV-1 RNA阳性,阳性率为87.3%(48/55),对48份PCR产物进行测序,其HIV-1蛋白酶编码区(PR)和逆转录酶编码区(RT)均测序成功。HIV-1主要流行毒株为CRF01-AE,占49.1%;其次是CRF07_BC和B亚型毒株,各占27.3%和9.1%;CRF01-AE/B重组毒株占1.9%。结论河北省内HIV-1流行亚型日益多样化,应加强对各类人群的干预和监测,采取有效措施切断HIV传播途径或不同途径间的交叉传播,特别是控制性传播上升的趋势成为当务之急。

Objective To analyze the distribution of HIV- 1 gene subtypes among HIV- 1 patients with anti- HIV therapy.Methods The gene in pol region,and the gene in encodeing protease and reverse transcriptase were respectively amplified with nested polymerized chain reaction（ N- PCR） and sequenced,and compared with international reference strains. The N- J phylogenetic trees for our sequences and reference sequences were constructed using Mega 5. 0,with which HIV- 1 subtypes were analyzed. Results Among 55 samples,48 samples were HIV- 1 RNA positive and sequenced successfully in PR and RT,and the positive rate accounted for 87. 3%（ 48 /55）. CRF01- AE was the dominant strain of HIV- 1,accounting for 49. 1%,followed by CRF07_BC 27. 3% and subtype B 9. 1% and CRF01- AE / B accounted for 1. 9% in Hebei. Conclusion With more and more subtypes of HIV- 1,it is necessary for us to take measures to prevent and control HIV- 1 prevalence for populations in Hebei. It is urgent to take effective measures to cut off the transmission route of the HIV or the cross infection in different ways,especially to control the increasing trend.