山西小麦品种(系)多酚氧化酶基因的等位变异分布

Allelic Variation of the Polyphenol Oxidase Genes in Shanxi Wheat Varieties(Lines)

为了明确山西小麦品种(系)多酚氧化酶(PPO)基因的等位变异分布,选育PPO活性低的小麦品种。利用PPO基因的功能性标记PPO16、PPO18和PPO 29对121份山西小麦品种(系)进行分析。结果表明:Ppo-A1a和Ppo-A1b基因型的品种(系)分别为56份和65份,频率分别为46.3%和53.7%;Ppo-D1a和Ppo-D1b基因型的品种(系)分别为50份和71份,频率分别为41.3%和58.7%;两基因组合类型PpoA1a/Ppo-D1a、Ppo-A1a/Ppo-D1b、Ppo-A1b/Ppo-D1a和Ppo-A1b/Ppo-D1b的小麦品种(品系)分别有43份、50份、37份和56份,分布频率分别为35.5%、41.3%、30.6%和46.3%。本研究在山西小麦品种(系)中检测出37份低PPO活性基因Ppo-A1b/Ppo-D1a组合类型的品种(系),为常规育种获得低PPO活性的小麦品种提供依据。

In order to clarify the allelic variation of polyphenol oxidase （PPO） in Shanxi wheat varieties （lines）, and select and breed the wheat varieties with low activity of PPO, a total of 121 Shanxi wheat varieties （lines） were tested with the functional markers PPO 16, PPO 18 and PPO29 of PPO. The results showed that the wheat varieties （lines） with Ppo-Ala genotype and Ppo-Alb had 56 and 65 accessions, respectively, and the frequencies were 46.3% and 53.7%, respectively. The wheat varieties （lines） with Ppo-Dla genotype and Ppo- Dlb had 50 and 71 accessions, respectively, and the frequencies were 41.3% and 58.7%, respectively. The wheat varieties （lines） with two-genotype combination types Ppo-Ala/Ppo-Dla, Ppo-Ala/Ppo-Dlb, Ppo-Alb/ Ppo-Dla, Ppo-Alb/Ppo-Dlb had 43, 50, 37, 56 accessions, respectively, and the frequency distributions were 35.5%, 41.3%, 30.6% and 46.3%, respectively. 37 accessions of the wheat varieties with low activity of PPO （Ppo-Alb/Ppo-Dla） were detected, which provided the basis for obtaining the wheat varieties with low activity of PPO in conventional breeding.