干扰素-α/β/γ单核苷酸多态性与中国汉族人群慢性HBV感染的相关性

Association between interferon-α/β/γ gene single nucleotide polymorphisms and chronic HBV infection in Chinese Han population

目的探讨干扰素-α/β/γ(INF-α/β/γ)基因单核苷酸多态性(SNP)与中国汉族人群乙型肝炎病毒(HBV)感染的相关性。方法应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法检测206例自限性HBV感染者(对照组)和210例慢性HBV感染者(病例组)INF-α/β/γ基因上7个SNP位点,比较两组间基因型和等位基因频率。结果对照组和病例组在性别分布、INF-γ基因上的rs11177916位点多态性差异均有统计学意义(P<0.05),而在INF-α/β/γ基因上其余6个位点多态性差异均无统计学意义(P>0.05)。结论男性患病率高于女性,具有INF-γ基因上rs11177916位点TT基因型及T等位基因的人群发生乙肝慢性化的几率低,有利于乙肝病毒的清除。

Objective To explore the association between interferon-α/β/γ gene single nucleotide polymorphisms（SNPs） and chronic HBV infection in Chinese Han population. Methods PCR-RFLP was used to detect seven interferon-α/β/γ gene single nucleotide polymorphisms loci in 206 cases of self-limiting HBV infection（control group） and 210 cases of chronic HBV infection（case group）. Genotype and allele frequency of two groups were compared. Results The gender distribution and gene polymorphism in rs11177916 site of INF-γ of the two groups had significant differences（P〈0.05）; the other SNPs in interferon-α/β/γ gene had no significant differences between two groups. Conclusions The morbidity in male was higher than that in female. The results suggested that the cases with INF-gamma rs11177916 on gene loci TT genotype and T allele were easily and spontaneously recovered from HBV infection,and less chance to become chronic HBV infection.