MTTP基因rs1800591位点多态性与非酒精性脂肪性肝病的相关性研究

The association between polymorphism rs1800591 in MTTP and non-alcoholic fatty liver disease

目的研究微粒体甘油三酸酯转移蛋白（MTTP）基因rs1800591位点多态性与非酒精性脂肪性肝病（NAFLD）的关系。方法采用聚合酶链式反应（PCR）-限制性片段长度多态性检测219例NAFLD患者和210例正常对照组MTTP基因rs1800591位点基因型和等位基因。应用方差分析、卡方检验、非条件Logistic回归分析等方法判断NAFLD患者与正常对照组的各项指标。结果NAFLD组与对照组两者rs1800591位点的基因型分布和等位基因频率差异有统计学意义（χ2=9.007,P=0.011;χ2=9.599,P=0.002）。经混杂因素校正后,非条件Logistic回归分析结果显示,与TT基因型相比,GG＋GT基因型发生NAFLD的风险比值比：OR=1.623,95%CI为1.070~1.648,P=0.030。等位基因G携带者患NAFLD的风险是T等位基因的1.521倍（OR=1.521,95%CI为1.089~2.123,P=0.014）。TT基因型与GG＋GT组ALT、LDL、TG的差异有统计学意义（ALT：P=0.001;LDL：P=0.008;TG：P=0.024）。结论青岛地区汉族人群中MTTP基因rs1800591位点多态性与患NAFLD的风险有相关性。

Objective To study the association between the microsomal triglyceride transfer protein （MTTP） gene and hereditary risk of non-alcoholic fatty liver disease （NAFLD）. Methods Peripheral blood DNA from 219 patients diagnosed with NAFLD and 210 control subjects were used to determine the MTTP genotype by polymerase chain reaction （PCR） and direct sequencing. Inter-group differences and associations were assessed statistically using t tests, Chi square and logistic analysis. Results There were significant differences in the frequencies of rs1800591 genotype and allele between the NAFLD and control subjects 052 = 9.007, P = 0.01 I; Z2 = 9.599, P = 0.002） . Logistic regression analysis adjusted for confounding factors showed that the OR of carriers with the GG ＋ GT genotype of rs1800591 developing NAFLD was 1.623, （95%CI： 1.070- 1.648, P = 0.030）, compared with those with TT genotype. Also, in contrast with T allele, there was an increased risk of NAFLD for rs1800591 G allele （OR = 1.521, 95%CI： 1.089-2.123, P = 0.014）. The levels of ALT, LDL and TG were higher in GG ＋ GT genotype than that in TT genotype, with significant differences. Conclusions The polymorphism rs 1800591 in MTTP is related to the risk of NAFLD.