香烟烟蒂水浸泡液的致突变作用

THE MUTAGENICITY OF CIGARETTE END IMMERSION

目的 :探讨香烟烟蒂水浸泡液对雄性小鼠骨髓细胞和睾丸初级精母细胞的遗传毒作用。方法 :用香烟烟蒂生理盐水浸泡液为实验材料进行小鼠骨髓嗜多染红细胞 (PCE)微核试验和睾丸初级精母细胞染色体畸变试验。结果 :各剂量组小鼠骨髓嗜多染红细胞微核率分别为 3.0‰、5 .6‰、11.3‰ ,明显高于阴性对照组 (2 .0‰ ) ,并有明显的剂量 效应关系。各剂量组小鼠睾丸初级精母细胞性染色体单价体 (4 .6 %、5 .0 %、6 .6 %)和常染色体单价体 (4 .4%、8.0 %、10 .4%)均显著高于阴性对照组(2 .4%、2 .6 %) ,并有明显的剂量 效应关系 ,而染色体结构畸变率与对照组差异无显著性。 结论 :香烟烟蒂水浸泡液引起雄性小鼠骨髓PCE微核率增高 ,并诱发睾丸初级精母细胞染色体数目畸变。

Purpose: To explore cigarette end immersion-induced mutagenesis in bone marrow PCE and primary spermatocyte. Methods: Micronucleus test in bone marrow PCE and chromosome aberration assay in primary spermatocyte were undertaken in mice exposed to the immersion. Results: In the low, middle and high dose groups, the mean frequencies of micronuclei (3.0‰, 5.6‰, 11.3‰), the mean frequencies of sex chromosome univalent (4.6%, 5.0%, 6.6%), and the mean frequencies of autosome univalent (4.4%, 8.0%, 10.4%) were higher than those in the control groups (2.0‰ in micronucleus frequency, 2.4% in sex chromosome univalent and 2.6% in antosome univalent). No significant difference in the frequencies of structural aberration was observed between the treated and the control groups. Conclusion: Cigarette end immersion increases the frequencies of micronucleus in bone marrow PCE and induces numeral aberration of chromosomes in primary spermatocyte.